Detailed Locus Information for CNAG_00073
| Species | Cryptococcus neoformans var. grubii H99 | ||||||||||||||||||
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| Gene Annotation | nuclear mRNA splicing protein | ||||||||||||||||||
| Gene Symbol | FAR8 | ||||||||||||||||||
| NCBI Protein ID | XP_012046507.1 | ||||||||||||||||||
| UniProt ID | J9VKI6 | ||||||||||||||||||
| KEGG Linkout | N/A | ||||||||||||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | ||||||||||||||||||
| Functional Annotation |
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| Amino Acid Sequence | MNRAVQRSQLFSPASQQPRSQYDPQNPGMQHRGPAGPVPQQQYQQYQQYQQPQQQGQQQQQQQQQLPQQGPQSTNGTADGASGSGSTPGQEMNLASVLHYLQSEWRRWERDRNEWEIERAEMRARIALLEGQRRSAENLKVDLLRRVKMLEFALRQERTKRVGAGGKLNSVPPTRLAALQDEDRLSTGDKEGSGSECSQEDIVEKPTKVNGIHSAAISKSSTIMSRSQPAEANQWKSIGVAPRDPKARARSREYLKQCLQEISYLTSPGALNPLPPYPPVDPSVLPQPEVSHDPNHPEVDIYERPRKELSEESVQPLHTRKRNDEAAESKETAGSTSVPANDQMNGEKDKSIPSIPAPALPPGPSASFPTSQPMEKSVSPSPLAFHRPGLDPALLPSSDNNRPPSSPSPRTVNLPEISTTKSEDEPQSLSAGKDSDEPGKQLLTAIYRPDSKTAWREELKAANEEAEKAKEERARKVPDQSEDDRLTTLSLNTEEEEVKADDSIDKIWVSKRSLKSHLDIVRAIAFAHGPGIMLATGGDDCTVKVWSVDSASIISHRHTTQEIEPIMTLRGHTAAITSVTISNALSIIFSASLDSTIRLWQLPAHNHDPYATYNPSVAVQTLEGHTESVWDICLLPPQEVSQPGKEAIEGRLVSASSDGSVKLWERSGSSPSASNWKLEKSFSSFGDGVIPTCLAVFNLDFGKVLVGTSDGKARLWDVDAGEEVRLFGGEGQGADSQVNAILSHPTLPAIVTAHEDGYLRFYDVKSSSTTPTHTVLAHPAPITSLALSPSSPTCILTSSVDCTVRLWDLTKKTSIQELAGHRGRADEGVCAVASHPELPVIGSAGADGVVRLWGLA | ||||||||||||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| FAR8 | B13314 CNAG_00073 5’ flanking region primer L1 | TCGTCCTTCGCTTGAAAACT |
| B13315 CNAG_00073 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACGTTGCTGACCTTGCTGTTGA | |
| B13316 CNAG_00073 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGCCCAACGTGCATCCTTACTT | |
| B13317 CNAG_00073 3’ flanking region primer R2 | TCTCGACCAGTTTGCTGATG | |
| B13318 CNAG_00073 diagnostic screening primer SO, pairing with B79 | CCCAAGGAATCTCGCTTGTA | |
| B13319 CNAG_00073 Southern blot probe primer PO | TCTCCCATCTTCTCCATTCG | |
| B14380 CNAG_00073 diagnostic screening primer SO2, paring with JOHE12579 | CTCCTAGCTTCCCCGTCTCT | |
| B14381 CNAG_00073 Internal_LP | CTGTCCCGATTTGACCATCT | |
| B14382 CNAG_00073 Internal_RP | AGGGGCTGGAATAGAAGGAA | |
| B5522 NAT#230 STM primer | ATGTAGGTAGGGTGATAGGT | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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