Detailed Locus Information for CNAG_01016
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | vacuolar membrane protein | |||||||||
| Gene Symbol | SEA3 | |||||||||
| NCBI Protein ID | XP_012049093.1 | |||||||||
| UniProt ID | J9VNW9 | |||||||||
| KEGG Linkout | N/A | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MLSPANIPLPISPALTASRRSSASSTPFQPSPLTALWPRSVAPARAQEDDDPFKEGATVSVNQPVGSISISPSSRDVCLASRKGLYILDLANLNNAPRFVPQGGAWQIADVQWSPHPATSNLILSTSSQKLLVWDLAAPRPLLKSFDAHSRAITDINWHALNPNLMATVSMDAGIRGWDLRCFDRPVMRLCDWGAAGTQVKWNRRHDHLIATAHGKIVHIWDDRKGSVPVTTIHAHDAKIYGIDWDRQYRHKLVTCSLDKTVKFWSVPELGGSSDSSMVYHSSLPPLSSPSYTISTHYPVWRARNLPFGRGVLALPQRGEKALEMFGMDDPAPVERFAGHENVVKDFVWRMRGGDDTSFDDREFQLVTWSKDRTLRIWPVSKELEERVGWQFGAPIKMLVSRRGAPDVTYAKDPSNIDIDTPRLPPPIVNLHPHNPSNLTRQKSARPEAQPGMTRGGNKVRSMDQLEWLTKVVKNAPSPESSLMSSRMASVSRTRRPGSRAASRLDSRSASPEGRQEWKNLKDEVVAVSRMYPRPKINFEKIDLAHHKLTISMQGPWANGDRQAFMRIHWSFPQNYPYGPEIPTFELERNPTVSPITRQTIVMNIKEMRAHNKQCLVETTGYLLGLHERQGRRRGMDEESDSESERGEEKRRDDVIPELMLRKTCGATFGPNGQLVCFFPKQVTLPRTRNFSRSPSMTRENPSPMLKAISALTRLQNPHQRAVLRYKPRARRLDPISSPLPPPAGSTMTIHDVSYLGQPNVHLAKMYGMSVEANMSYALEAKRLDHADVWATVRGILADPPPPYTQLPQLDEKQESVRRERLIWEKGMERKKRVVDRLFTNLMAQKDVQMLALLSCILLEYTRSMYIPPVAESVTSHSPLQDYFNLRFPPPRPTLNTIPVRRRTGSSVIPVSPTNSSSFRTSGWSQILNPSGISLRGALTPIEHTSFSDMPFSRTILGTSYEEQSSPSGMAIPALKQSDSPKPIPGDKAKAFPTSSMSPPPIVTPLRSSGPEKSLYTGGEPRSHKVSFGSASPIARGGRSQSSTGYVGGGLGSAVTTGPNTPDGERRVAVRSGGVRLDFPKDESSTPSLLPQDMLPLCEAWKLSYADFLLRHHLLGVRMTLLSYRFITSSASAAVSAASGKSSGIVRAGVGETEKIDEGQSVIRVCVPCSGTPSGTCPSCNRQPQKAVCSYCRVPIKGISMGCTICAHKFHAKCFQRYFLSPVTTPMTCPACSCSCLAHRGISTPLYTVATLPKQSPNITRGFDRGKIPSWNAPFARSRSVPSQGDRGQNSPEEGKGRLTYASLVKLGAIRENFGLGFADGASSGTASALGLNADGDGDEVSTDRDRTVREQRGDHRGDGLLSRARWGGEGLLHWGSTTHHQ | |||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| SEA3 | B13951 CNAG_01016 5’ flanking region primer L1 | ACGCCTCGTTCGATATGCT |
| B13952 CNAG_01016 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACAGATCGGCAGGGGGATGT | |
| B13953 CNAG_01016 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGTTTACTGTCGCGAGCAAGGT | |
| B13954 CNAG_01016 3’ flanking region primer R2 | GGTGGCCTCGATAGTGTTTG | |
| B14418 CNAG_01016 diagnostic screening primer SO, pairing with B79 | GCTGCTCTGTGGAGGTTTTC | |
| B14564 CNAG_01016 diagnostic screening primer SO2, paring with JOHE12579 | ATAACGGTCGCCGTGATTAG | |
| B15998 CNAG_01016 Internal_LP | GACGACAGAGTGCGTTGAAA | |
| B15999 CNAG_01016 Internal_RP | CTTGGACCAGGTCACCAACT | |
| B16417 CNAG_01016 Southern blot probe primer PO | CGTGCACCTGTGTTGCTTAC | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG | |
| B6348 NAT#146 STM primer | ACTAGCCCCCCCTCACCACCT |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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