Detailed Locus Information for CNAG_01262
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | guanine nucleotide-binding protein subunit beta | |||||||||
| Gene Symbol | GPB1 | |||||||||
| NCBI Protein ID | XP_012049425.1 | |||||||||
| UniProt ID | J9VT59 | |||||||||
| KEGG Linkout | N/A | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MQDIQDKIAAARREADGLKDKLRAARDQTADTSLRAMATDTPPLPRITLKARRTLKGHLAKIYALHWAADTRHIVSASQDGKLIVWDAYTTNKVHAIPLRSSWVMTAAYAPSGNLVACGGLDNVCSIYSLRGAGPGAPGAPGGQVKVARELSAHSGYLSCCRFINDRQIVTSSGDMTCMLWDIEQGTRTMEFNDHTGDVMSISLAPNANLFVSGACDATAKVWDIRTGKAVQTFTGHESDINAVQFFPNGDAFATGSDDASCKLFDLRADRELNSYAHDNILCGITSVAFSISGRVLFAGYDDYNCNVWDTLKGERIGVLAGHENRVSCMGVSGDGVALCTGSWDSLLKVWS | |||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| GPB1 | B20258 CNAG_01262 5’ flanking region primer L1 | GGGTTTTGTCTCGGTCGTAA |
| B20259 CNAG_01262 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACCTCGCAGCTTGTCCTTCAG | |
| B20260 CNAG_01262 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGCATGGATTACGGATGCAACA | |
| B20261 CNAG_01262 3’ flanking region primer R2 | CCCATCATCGCCTCTATCAT | |
| B20262 CNAG_01262 diagnostic screening primer SO, pairing with B79 | ACATGGTTCCGTTAGGCAAG | |
| B20263 CNAG_01262 Southern blot probe primer PO | GCAGGGAGTAGATGGAGCAG | |
| B20336 CNAG_01262 Internal_LP | GCCTACACCACCAACAAGGT | |
| B20337 CNAG_01262 Internal_RP | CACCCATGCAGCTGACTCTA | |
| B20338 CNAG_01262 diagnostic screening primer SO2, paring with JOHE12579 | TTTCGGTTTTCGTCAGTTCC | |
| B5502 NAT#125 STM primer | CGCTACAGCCAGCGCGCGCAAGCG | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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