Detailed Locus Information for CNAG_01337
| Species | Cryptococcus neoformans var. grubii H99 | ||||
|---|---|---|---|---|---|
| Gene Annotation | WD-repeat protein | ||||
| Gene Symbol | BUN62 | ||||
| NCBI Protein ID | XP_012049478.1 | ||||
| UniProt ID | J9VKN4 | ||||
| KEGG Linkout | N/A | ||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | ||||
| Functional Annotation | |||||
| Amino Acid Sequence | MELYMYTPPVYMAPMRPLVKPAPSVPIVKEGPIPELFQAPEGQYNLADPGSMFPMLGNTSPNIASELRLACQPGPFLGGPGFGIPPTPQQTSPNAASASSTCFGATQINGKWEPIYPTRMSWVTVQFPSKTGDRGLGGLLGKGGKGDAAAFLPPAAADGRYPTKSSYSSSSSSSSPISSEPQSVPFAMSPPTVQSKWPFAKTINAIPRPKTNMRNSTSEFVQRVIGLDSASKYLAEKGKTVSEVVKWGCWHMGRQWAWGDFGKQANETEKKKKDALVKVLFSTPLTCTAIINQTAGVDRLDVIIGFETGDLLWLDPILGKYTRLNKNGVLNSSRVVAIYPDPRQPTHFLALFADYTILRFNISLEDPINAANITSRPWDVFFDRVLLATTNGPDPSLSSDSGTGLYLDREQDVELLKWKNEDWVAGVEAERAKDKNAIVFTGRNPVAALKIGGARINALAYSPDGGKLASVSSDGLLRVIDTSEERITDTFSGYYGGLNCVAWSPDSRLIAAGGEDDFVTLFSTGRDARTIARCQGHSSYITCIAFDPQSNNPSSRAYRFVSVGEDGKLLFWDYSPATVHKPRQHHANGSAQRDAAASSMTVNIVDPSRSHTPAERISGRFHPAPSRKSIPTLQPVMSKTVDVTILTGVYCLPDAIATVSRQGVARFWMRPSSRPPA | ||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| BUN62 | B13308 CNAG_01337 5’ flanking region primer L1 | TAGATATTCGGCGGAGGAAA |
| B13309 CNAG_01337 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACGAATACCAAAACCGGGACCT | |
| B13310 CNAG_01337 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGGGCGTATATTGCCTTCCAGA | |
| B13311 CNAG_01337 3’ flanking region primer R2 | GCGAGTCACATCAGATTCCA | |
| B13312 CNAG_01337 diagnostic screening primer SO, pairing with B79 | TTTAACTCGTCTGGGGATCG | |
| B13313 CNAG_01337 Southern blot probe primer PO | TCGGGGACGATGAACTAGAG | |
| B22923 CNAG_01337 Internal_LP | AATGCCGCAAACATTACCTC | |
| B22924 CNAG_01337 Internal_RP | GATGTTGTCGTGGTTTGTGC | |
| B22925 CNAG_01337 diagnostic screening primer SO2, paring with JOHE12579 | CGTGAACGTATGAAGCGAGA | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG | |
| B5965 NAT#295 STM primer | ACACCTACATCAAACCCTCCC |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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