Detailed Locus Information for CNAG_01547
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | WD-repeat protein | |||||||||
| Gene Symbol | AIP1 | |||||||||
| NCBI Protein ID | XP_012052591.1 | |||||||||
| UniProt ID | J9VTG9 | |||||||||
| KEGG Linkout | N/A | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MSYKAGSVYPCNPATSRSESTKLGVDPKGEKLVYTNGRAVVIRDLNHVGLSHVYTEHTQNTTVARISPSGYYCASADIAGNVRVWDVTQPENIIKLATRPLGGKINDLAWDGESKRIIVGGEGKDKFGAAFFMDSGSSCGEIAGHAKPITALSIRQQRPFRAISGSDDNSLIFHTAVPFKYDKMINTHTRFVRDVAFSPDGGLFASVASDGKMFFYEGKTGEVKGEADRGGATASLMACSWAPDSSRIATAGTDGIVAIWDSSTLKTIQSYNVGSDVAAQQNGVVYANPNIVVSASLSGTLNIFDIRESTSTWRTLHGPTKAITASAISDGNGNDTTFYAGSFDGTMKKFEIGEAYGEKEGTCDEIGGTGHSARIAAISANGKDKVWSAGWDDKVTAIEGSQFTSSPIPTKAQPTSIAATPDSVYIATASGVEVNGPSPITLVTSPTSAVAAYPGPNSDLVATGSGKIVTLFSISPQAVLATFDDNKGDVLSLAFSPDGKYLASGDATGRIILIDVEKKETAVSSKWTFHTGRVVGLAWANDSKRLASAGLDENIYIWDTKKQLKNISIKNAHPGGANGVAWVDGNTRLVSAGADGCVRTWTVPEL | |||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| AIP1 | B13767 CNAG_01547 5’ flanking region primer L1 | CCCAGTGCAAAGATCGAGAA |
| B13768 CNAG_01547 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACGTCAGCCCACCTGCTTTGTA | |
| B13769 CNAG_01547 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGTGGGTTGATGGGAATACCAG | |
| B13770 CNAG_01547 3’ flanking region primer R2 | GATTACGCAAGCGGTCATTT | |
| B13771 CNAG_01547 diagnostic screening primer SO, pairing with B79 | AGCAAAAACATCGCCATACC | |
| B13772 CNAG_01547 Southern blot probe primer PO | TCCACATTGGTCAGCGTAAC | |
| B14317 CNAG_01547 diagnostic screening primer SO2, paring with JOHE12579 | ACCCAGGTCGTTGTTGTCTC | |
| B14318 CNAG_01547 Internal_LP | ACGGAGGATTTGGACTCCTT | |
| B14319 CNAG_01547 Internal_RP | TGCAACGTCTCGAACAAAAC | |
| B5493 NAT#5 STM primer | TGCTAGAGGGCGGGAGAGTT | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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