Detailed Locus Information for CNAG_00416
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | f-box/wd-repeat protein lin-23 | |||||||||
| Gene Symbol | WDP9 | |||||||||
| NCBI Protein ID | XP_012046304.1 | |||||||||
| UniProt ID | J9VIY2 | |||||||||
| KEGG Linkout | N/A | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MPGSPPLQRHVSTKAELLQKALTLPLELRRELATSLLWSMPRNEVLALNERLNTMLQKDILGLLPPELAFLILARLDLDDILKCSAVSRTWRNLCNKQALWALLCATNSPPIRPLPISWAELTTQYSLPTPTDDIEDPTFDISLSDDRLSNHYDVIDGQGGMGGGGGIDPLGMSGGLRRNVWERGGAGSGEGLPVHLQRSVVNVQGFRKKEEPPIASHLAVPSANPQANFKHLYIVHHILEQRMKTPRPIGMANYMPSPPEEVKINKPLSLPPVPRPRTIDAISSVKYGGLPGHSEAVYSLSLISHSMRINMLQACPDCHVQLGTPSNLSGTTEGQFQLSVVETLNGTGASPRRILPNLSQAGGKRNLVIGRDWLLSGSRDKTLRLWQLSPAPRVVKVFHDGHTGSVLTHAIVKVPCLSFASSPQSRFSKLEIGPLTKEVKERMKVMAVSGGSDGKICLWDVEGGNGEPEKCVKAHEDSVLCVRADDERVVSCSKDKTIRLFSIHTLEPLLVIGGTGDLNLHRGAVNAVGLSKDYIISASGDKTLRVWSIKSGALLACIEGHHRGIASIDFSPLPPFPINLPDGQIFRGLIVTGSSDASIRTFYLIEDRIPDLENLEMGHSMVTSPTDGSEDGHFQANTYGIPTELTEEGHKVILVPQGSFFSPCICPPGLSRMEGGVCRRCFNRGHLDLVRTVHFGKGVILSGSYDATVKIWSPTGEIIADLHGAHTGRVFSVVGDRMRVVSSGLDCRINIWDFSEGLDTSFVAP | |||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| WDP9 | B11702 CNAG_00416 5’ flanking region primer L1 | ACCCAGGTCTTCATGTCCAG |
| B11703 CNAG_00416 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACACGCAACATACCTCGTCTCA | |
| B11704 CNAG_00416 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGGAATGGAGGGAGGTGTCTGT | |
| B11705 CNAG_00416 3’ flanking region primer R2 | TAGGACCGATGTAAGTGCCC | |
| B11706 CNAG_00416 diagnostic screening primer SO, pairing with B79 | TCAGCATTATCAACCACCC | |
| B11707 CNAG_00416 Southern blot probe primer PO | AGCGGGAGGAAATCTTATC | |
| B22926 CNAG_00416 Internal_LP | CTTCACATCTCGCTGTTCCA | |
| B22927 CNAG_00416 Internal_RP | CCCACTCACTGCCATTACCT | |
| B22928 CNAG_00416 diagnostic screening primer SO2, paring with JOHE12579 | GACGAAAGTTGGCCAGGATA | |
| B5529 NAT#288 STM primer | CTATCCAACTAGACCTCTAGCTAC | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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