Detailed Locus Information for CNAG_03681
| Species | Cryptococcus neoformans var. grubii H99 | ||||||||||||||||||
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| Gene Annotation | peptidylprolyl isomerase domain and WD-repeat protein 1 | ||||||||||||||||||
| Gene Symbol | WCP1 | ||||||||||||||||||
| NCBI Protein ID | XP_012047385.1 | ||||||||||||||||||
| UniProt ID | J9VIE9 | ||||||||||||||||||
| KEGG Linkout | N/A | ||||||||||||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | ||||||||||||||||||
| Functional Annotation |
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| Amino Acid Sequence | MSEGAGSSSKLGKRARDGESTSPAPEDANNNGSEVPEMPPADMEDSSDDEIGPMPGGDIVISNGRKKKRTVLPHEKLYLASLPDTDRYYKSFMHREPLNSITMTRTNFVITTSIDGHLKLWKKQEQGIEFVKHYRASLKAIVGVSASDDGKLFATVSEGGEGRVFDVVNFDMINILKFPYTPKACCWIHEPGAGQPLLAVSDAASPTIRIYDGRGDGKPLYELTKLHRAPVHLMVYTAKYDCVVSADEDGFVEYWQPSEPWDLPSVPGLWQYKSSTDLFHFKKSKTIPTSLTFSPNSSHFVTLALPSRAVHIFNFLTGKLTRTYDESLTAVMEMQQAGTAVFKLDDMDFGRRLAAERELDRGESGPGGLLRTANAVWDESGNFVLYPTMLGIKVVNAVTNKVARVLGKDETLRFLNIALYQGAPAKKGVTTIQMAASANPLLQDKASRDPHLFATAYQKQRFYLFARSDKEESKGERDVFNERPTREEQTVAVPVEEKKRPSAKRCTIHTTKGDISVQLFPDEAPKTVENFITHARNGYYNGTIFHRIIKKFMIQGGDPFGDGTGGESIWGGTFEDEISPRLRHDRPYTLSMANAGPGTNGSQFFITTVPCQWLDGKHTVFGRAVGGLDSVDAIEDAKTDKNDRPFEDISISSITVE | ||||||||||||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| WCP1 | B17145 CNAG_03681 5’ flanking region primer L1 | CTCGGGGAAGATGTACGAAG |
| B17146 CNAG_03681 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACAGAGGTGCTTTCACCGTCAC | |
| B17147 CNAG_03681 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGCGTAGTGCTTTAGCGTGTTTG | |
| B17148 CNAG_03681 3’ flanking region primer R2 | TACCCTTCTTGACGGTTCCA | |
| B17149 CNAG_03681 diagnostic screening primer SO, pairing with B79 | GCATGCTTTGTGACCAAATG | |
| B17150 CNAG_03681 Southern blot probe primer PO | CGGTCGGTATCAGGAAGAGA | |
| B17501 CNAG_03681 diagnostic screening primer SO2, paring with JOHE12579 | GCGTCGATTCTTCCCAAATA | |
| B17502 CNAG_03681 Internal_LP | TGCTCTATCCAACGATGCTG | |
| B17503 CNAG_03681 Internal_RP | GCTTCGTCAGGGAAGAGTTG | |
| B5518 NAT#218 STM primer | CTCCACATCCATCGCTCCAA | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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