Detailed Locus Information for CNAG_03908
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | WD-repeat protein | |||||||||
| Gene Symbol | WDP8 | |||||||||
| NCBI Protein ID | XP_012047541.1 | |||||||||
| UniProt ID | J9VGZ0 | |||||||||
| KEGG Linkout | N/A | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MSSGGQTNAAGPSRQHSLTAESRSLTTARQKEKAPQLEDVVLEEGLFEQEIDLLIGQYLEARGASKAARIWDEQVLGRSANGSTRKEHIEDVERAILDGDWGSIENLIARPGLLRIQTQKAFLYLVYRQQFLEHVENRESQKAFNLLQKRLKALEHYQPVPYDFYSLSYLTSASTVHDAPTFRDWAGVGPERDRLVGVWKELMDAERDGGVDERRYIPPDRLRTLLKQAAAWQVGRVGKRTDDIVKIPTLLQDYRPLEIPSKLLHLIEGHLANVKCVDLIGSTAQYVVSGSSDCTLRVVSTEDGSLSHILSGHSSRVWSCASSPSGETIASSSGDGTIRLWSASKGDCLGVLVGDGGDVYNVRWRPNREDQVVSASYDRILRSWDIETGKQLRTFSGHSQSTLAIAYDSTGNTIASGSKDKHVRLWDAVGGVCTNTMTDCLGEIASVEFDNEGKYLLVGCKDNSNRLWDLRMQRSVYRYTGHQNTSKNLVRCSFACDTSLVISGSEDGSVYIWDRESNSNDRLSNSLVPSTRHNELLAPERKGSGSDLTDFNIHASSPAYYPPRDGIRNRTSFARRGEITVRPSKVLMGHGEGAVFEVRWKEGKMVSAGEDGSVGVWGVEAN | |||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| WDP8 | B13785 CNAG_03908 5’ flanking region primer L1 | CGAGTCTAGTCGGGCTGTCT |
| B13786 CNAG_03908 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACGTTTGTCCTCCGCTTGACAT | |
| B13787 CNAG_03908 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGTGCAGGTGAAGATGGTTCTG | |
| B13788 CNAG_03908 3’ flanking region primer R2 | CAACAAAGCGAAATGAAGCA | |
| B13789 CNAG_03908 diagnostic screening primer SO, pairing with B79 | TTGCTGGGAAGCTTGATTCT | |
| B13790 CNAG_03908 Southern blot probe primer PO | AAAAAGCCCTTCCTCCAAAA | |
| B14422 CNAG_03908 Internal_LP | GCGTAGACACAAACGCTTCA | |
| B14423 CNAG_03908 Internal_RP | CAACGCACGAGATTCTTTGA | |
| B14424 CNAG_03908 diagnostic screening primer SO2, paring with JOHE12579 | TGGAAGGGAGTTTTGTCCTG | |
| B5519 NAT#219 STM primer | CCCTAAAACCCTACAGCAAT | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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