Detailed Locus Information for CNAG_03940
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | elongator complex protein 2 | |||||||||
| Gene Symbol | ELP2 | |||||||||
| NCBI Protein ID | XP_012047563.1 | |||||||||
| UniProt ID | J9VP43 | |||||||||
| KEGG Linkout | N/A | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
|
|||||||||
| Amino Acid Sequence | MRITSEYISIGANRSSSCAACSSSGLLFFGAGKFIALWDSSSDRGVHATLRGHKGQVTTVKLLPDGRLVSGDNIGEIRIWNSVRGEDEWECVMSWEAHRGGSISAIGVLASTGNLDDMVLTGGSDSLIKRWKIADKPEEVQKIDLKGKLPLDLEVGYLPGSEAPILAVGCTDRRIQIWTVRDGSFTRALSLEGHEDWVRCLSFTPYPSASSSSQDLLLASGSQDNFIRLWRVSSIEQEVASPSTGDEGLEMLDEFEKRLAGEAGGNVQISTKAHILGVQDGENTLRFNITLEALLVGHESGLTNVHWSPVPTSSSPTPLLLSTASDNSLIVWSPSSTSTSTDGIWVPTNRFGAIGGRGLSFYGAIWGKDGKSVMASGWNGGWEKWVESEQGWDVQRGLTGHHGDVQTVCWDPRGEYLLSVASDQTARIHAECNLPSISTPIWAEIARPQIHGYDMTDASFISPLRFVSGADEKVARVFDAPQGFVESLRSLGISKREAEEESRPKGATVPPLGLSNRALQKAPVAGDAVEKQGQNEAIISISHTFTSLPTEEELATSTLWPEVEKVYGHGYELVCAAASHAGDLIATASKATNAEHAVIRVVSASKWELVGEPLGGHSLTITSVSFSRDDNRILSCSRDRGWRVFERKVDGEGYVPLAGEEKAHARMVLDACWADERNDMFATASRDKTVKIWIPAVEDGSQWAAAETIKLTVASTAVAMINNGSDGYLLAVGKESGSIEVFAVAVNADGVKSDLLFTFDPRVSHVSAVNKLAWRNVGGVLSLASCSDDRSVRVYKVEL | |||||||||
| AlphaFold Structure with WD40 domains |
||||||||||
Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| ELP2 | B11119 CNAG_03940 5’ flanking region primer L1 | CTTGAAGTGGTGCTGTGGTG |
| B11120 CNAG_03940 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTAC ACGATGAATCCCAAAGTGCG | |
| B11121 CNAG_03940 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTG AGTGATGATCGAAGTGTCCGC | |
| B11122 CNAG_03940 3’ flanking region primer R2 | TGGCAATCACGACACCATTG | |
| B11123 CNAG_03940 diagnostic screening primer SO, pairing with B79 | AGTGGCGGTGGAATCGATAG | |
| B11124 CNAG_03940 Southern blot probe primer PO | CAGCTTGCCTTTCAGATCGA | |
| B12029 CNAG_03940 diagnostic screening primer SO2, paring with JOHE12579 | TGAGGAGAGCAGTGTGGATG | |
| B12030 CNAG_03940 Internal_LP | TGGGAAAAGTGGGTTGAGTC | |
| B12031 CNAG_03940 Internal_RP | GCTCGGCATTAGTAGCCTTG | |
| B5504 NAT#150 STM primer | ACATACACCCCCATCCCCCC | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
|---|---|
 |
|
| Mating Data | |
 |
|
| Virulence Factor Production | |
|
Capsule
Urease
Melanin
|
|
| Stress Response and Adaptation | |
|
Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
|
|
| Antifungal Drug Susceptibility | |
 |
|
| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
 |