Detailed Locus Information for CNAG_04117
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | hypothetical protein | |||||||||
| Gene Symbol | IQW1 | |||||||||
| NCBI Protein ID | XP_012051414.1 | |||||||||
| UniProt ID | J9VW48 | |||||||||
| KEGG Linkout | N/A | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MPRHVIRPLPKAFQPHRESHLHSDRTMWETLDRVQVLGDNQQGHYGCVNALSWSDDGQTLLSGSDDRRICIWQPDTTSHSSLSPHPLKLSETISTGHRANIFSAKFLPYANTPRIVSVAGDRDVRVYDVESLGRRMGEAGDWELDGVSGEGVTLLKCHKNRVKRIATENSPSLFLTVSEDGTVRQHDLRRPHSCSSECPEALFQAPRGVDLYSLSVSTVTPHIFAVAGTSPYAYICDRRMLPRQTPSWGPYIKSAGDVYCVRKLGLPDEEWETVSPRGRRRLFDGERHVSCVKMSGENADEVAVNFMKHSTSLFSIHDSPSSPSARSPSASSVIAPEIPSGRSRRSRSHGPTSVLPQSGAREGVENTDGPVVSERRTSIRSTGDSERGLDGRQIPTDGESASGRIEGLASLLERQIAEEEEEKEDNDGDGADSDHLRSFAQGFQHDSGDYNMVGPVQSLEERGDEMDYLMSADYLDLVRSDVDEDGGGEDNHSMQNPDRDRDNPEDGNDPLNEDDDDDEDEDEGDEDEAMFDDDEFFDSPIFGFPFGGHSSTAPREAYDNVETIHPRRMFKGARNVETVKDCNFLGTKSDKIASGSDDGYFFVWDKETGRLEGIWEGDGSVVNVMEQHPTLPLIAVSGIDNTVKMFSPIHNRPAPSISFNRSHMASTIVDRNTRPPRFVSGSIFESAALLQLLESRGVTVADLEDEPDGERECTTQ | |||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| IQW1 | B13957 CNAG_04117 5’ flanking region primer L1 | GTCGTCTGGTACCTGGCATT |
| B13958 CNAG_04117 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACCCTTTGGGAGTGGACGAATA | |
| B13959 CNAG_04117 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGGATTTGGAGGACGAACCAGA | |
| B13960 CNAG_04117 3’ flanking region primer R2 | CTCGGCTTGGACCTCTCTC | |
| B13961 CNAG_04117 diagnostic screening primer SO, pairing with B79 | CGTATTGGGAGAATCGGAGA | |
| B13962 CNAG_04117 Southern blot probe primer PO | GGTGAAAGGGAGGAATGTGA | |
| B14545 CNAG_04117 Internal_LP | CCCTGATGAAGAATGGGAGA | |
| B14546 CNAG_04117 Internal_RP | GGAATCTGCTGAATCCCAAA | |
| B14547 CNAG_04117 diagnostic screening primer SO2, paring with JOHE12579 | CTCGACCCATCGAGGTTAAA | |
| B5504 NAT#150 STM primer | ACATACACCCCCATCCCCCC | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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