Detailed Locus Information for CNAG_00516
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | peroxin-7 | |||||||||
| Gene Symbol | PEX7 | |||||||||
| NCBI Protein ID | XP_012046771.1 | |||||||||
| UniProt ID | J9VEA4 | |||||||||
| KEGG Linkout | N/A | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MRYLRLPLPPFAHNNLAFSPFYDHHLALASGSNFGLVGNGRVHVVKMDQQVAGGLGLVRSWDTADCVYDVAWSEIHENQIAAACGNGAIKLFDLALEGLPIQAWQEHTAEVTSIEWNNIEKALFVTGSWDQSVKVWNPSRQSSILTIPAHAGQIYSSTWSPHSPTIIATCASDGFIRIWDTRTLPSSIQEIFPPSAAPNPMSPSSAGEILSCDWNKYTPQLLAFSSQDGGVSTVDLRYISRNAEKMAVRLVGRHSLPARKVKWDPHNGTRLLSAGYDMTCRVWQTDLPPAAPLKELFNHQNHTEFVMAADWALFDPGLVASAGWDGDLHMYRV | |||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| PEX7 | B13749 CNAG_00516 5’ flanking region primer L1 | TTCCTTTGCTCCGTCCTCTA |
| B13750 CNAG_00516 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACAAGGGGAGAAGGCGAGATTA | |
| B13751 CNAG_00516 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGTTGTAATGGCTGCAGACTGG | |
| B13752 CNAG_00516 3’ flanking region primer R2 | TGAAGCTGTTCGTGTCCTTG | |
| B13753 CNAG_00516 diagnostic screening primer SO, pairing with B79 | AAGCTGTCAAGGCTGCTTTC | |
| B13754 CNAG_00516 Southern blot probe primer PO | CAATCCGCTGTATCCCAACT | |
| B14308 CNAG_00516 diagnostic screening primer SO2, paring with JOHE12579 | GAGTCAACCGTCGCTTCTTC | |
| B14309 CNAG_00516 Internal_LP | TCCCAGTCGACAGTCATCAA | |
| B14310 CNAG_00516 Internal_RP | CTTACCGCCATCTTCTCTGC | |
| B5496 NAT#58 STM primer | CGCAAAATCACTAGCCCTATAGCG | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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