Detailed Locus Information for CNAG_04158
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | protein HIR1 | |||||||||
| Gene Symbol | HIRA | |||||||||
| NCBI Protein ID | XP_012051625.1 | |||||||||
| UniProt ID | J9VRN3 | |||||||||
| KEGG Linkout | N/A | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MKVTKPNWVEHTVGEKKAKTAIYSISVHPDGTRLATGGLDHKVKIWSTLPILDMEAEKEEENPKLLCTMSSHTGSVLSVRWAHHGRFLATGSDDQVIMIWGLDPDGGGRLWGSDEVNVENWKALTRLVGHVADVVDLAWSRDDTMLASVGLDSTVWIWDGLTFERLRKLDLHQGFVKGVCWDPVGNYLATQSDDKTVKIWNTENWSLAETISKPFETSPQSTFFRRLSWSPDGAFIAASNAMNGPVFVAAVIDREGWASDISFVGHENTIQVAAFNPRLFFPEGEPKGRATASSMLALGANDFSISIWRNTLYKPLVVLKDIFGADLMDLCWSNDGYVLYGSSVDGSVCAIQFEPSEFTDLADFSATELVLREYDYKPKRAHQPLAVHSSAASITNGFGPSTTTSTHVNVLQPKKGKAKRRVDLSNGNVNPGPSASSSHQALRPPPPADPFSGPIQGFASPSTAQASTARMFEDAHRAFGPGNAGTSATSPRAGDKRKASGSYNGPTRGVRGRGMLVEHPVQQSEVQIIRAPMVAPSPSGTGLSKAYLPHPQVKSILRAQAIGNGNRGVYLEARNTSDPKGKNVFCYFADDEQRWIDYLPKAALAVTVTKQFCAAACEDGGLRVYSPAGRLILNMKLSGLVYDIHGEDKMLLIITMDCQVRVINVRNGKAFSPPSSIHHLLFPGSSSFRSFEITSCTVRPNGVPVIITSEPQAFAYDASLHEWTTIASPPIAGIQPLPGGPSGPLSVVDQVVAKSAPVTTAEKSNAPWLEESHVMSQFEMKIRATVLLDSKEEHRHWLLGYMKYLGDENFVERAEEVMRDLIGPVYHQLKPTGWEPKLLDVDKREIAVEALDVLSKTLQGKNVASVWYDVLDKIKADGASW | |||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| HIRA | B13917 CNAG_04158 5’ flanking region primer L1 | GGGGTTGAAGCCTACAAGCTA |
| B13918 CNAG_04158 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACGGGTTAGCGAGCACTCTTTG | |
| B13919 CNAG_04158 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGAGGGCAAAAATGTAGCATCG | |
| B13920 CNAG_04158 3’ flanking region primer R2 | GGCTAGCTGTCTTCCTGGTG | |
| B13921 CNAG_04158 diagnostic screening primer SO, pairing with B79 | CAAAGGGATTTCGCAGTAGC | |
| B13922 CNAG_04158 Southern blot probe primer PO | ATCAGCAAAGTTGCCCTGAC | |
| B14371 CNAG_04158 Internal_RP | CAAAGATGCGTCGTAAGCAA | |
| B14969 CNAG_04158 diagnostic screening primer SO2, paring with JOHE12579 | TAATTGCGGCTAATGGGAAG | |
| B14970 CNAG_04158 Internal_LP | CACCTATGGTGGCACCTTCT | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG | |
| B5965 NAT#295 STM primer | ACACCTACATCAAACCCTCCC |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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