Detailed Locus Information for CNAG_04170
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | WD-repeat protein | |||||||||
| Gene Symbol | CMR1 | |||||||||
| NCBI Protein ID | XP_012051428.1 | |||||||||
| UniProt ID | J9VYX0 | |||||||||
| KEGG Linkout | N/A | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MDEETAYELERQKTIAENRALLDSLGLDSAGVSSPFGSSPAPTSNKTKKPKQPKPAAPKKRKAPVIAADEGPRRRSGRIAGLEADGDAFKAKVEEEEKEREILRVVSRKEREQVMDVGKMVEDTPEDEIKDMEKYLQSIAELSNPRTYPAETVSAHEAYADSDTAVPLEVQRLKDAFKDMSLKGNTKVTNERVFSMCVHPEKTKTLVLVGDKYGQLGIWDALGPPMEKPENEDDTSGGMNAKEEDEYQEGRVWRVQAHAKNSISCMKVDPVNGSGLFSTAYDCSLRHLDFSTLQSTELFSFQDEDMLINHFDLLPGAQEAWMVDKNGGISHWDTRESKKESGRRRWVVQEEGRGAKLGGVSVNPLMPHLICTAGNDQHVRIWDTRHLFSISSHPVPIPVASLPATEQGETAPQNTHPTLESDYNTLTSYLASPQGKGLMRAKWQHGKSCSSAYWDPWGRRILTTSYDDHLRVFNVDPLVSSVNVNDNVNGNGFKPTKVVRHNCQTGRWLTILRAQWSLNMEYMPHFTVGNMKRTLDVVSATGEKIVALWTDDVTAVPTVTASHPNIVDRVVGGNTSGRIQLWSSGDNIYE | |||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| CMR1 | B17151 CNAG_04170 5’ flanking region primer L1 | GAGAAGGGGTGAGAGGGATT |
| B17152 CNAG_04170 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACCGCACTATGCTGTTTCCTCA | |
| B17155 CNAG_04170 diagnostic screening primer SO, pairing with B79 | AAATTTTCAGCCCACCCTCT | |
| B17156 CNAG_04170 Southern blot probe primer PO | CTCTTCCACCTTTGCCTTGA | |
| B17298 CNAG_04170 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGCTGTGTGCTGTCTCCAAATGA | |
| B17299 CNAG_04170 3’ flanking region primer R2 | CACATACGTCACCCCATTCA | |
| B17354 CNAG_04170 Internal_LP | TGCAAAAGAGGAGGACGAGT | |
| B17355 CNAG_04170 Internal_RP | TTCGGACATGTTGGTCGTTA | |
| B17356 CNAG_04170 diagnostic screening primer SO2, paring with JOHE12579 | GTCGTACACGGTAGGGCAGT | |
| B5531 NAT#296 STM primer | CGCCCGCCCTCACTATCCAC | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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