Detailed Locus Information for CNAG_04377
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | vacuolar protein | |||||||||
| Gene Symbol | SEA2 | |||||||||
| NCBI Protein ID | XP_012051511.1 | |||||||||
| UniProt ID | J9VS69 | |||||||||
| KEGG Linkout | N/A | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MLCVCTMNRFTNAFAAAIPTPSSSDAASLCPRDSPHVSPPAHPDTRHTAIYDDPRSPHPPAAPPRPAFLRHLTDQGPRPHAATPRVRSASRERDRSVRVNLMAAAATTARRGWGMSSATGTTRMNRMADGPDGMYAVGGGQYLRVFQVSDPCSGSSTPMIQEDPRERTLLARGQGGAAISEVANLWKSNWPVGKGVNDVDWGVAAWDHKLVTATPSGNFMLFDVEKGRLDREISSGSFRPLNCAQFCHQPSYCHMVLIGGADGNIKLLDLRTGDPPSRRPLRHSSAITSLCFSPNDASSFVIGLDDGTIKRYDWRMAGKHLGTVYGAHGSKAVMDLKWKPADDSVGGGGAGWLASAGANKIVQIWDMNQSWEKAPTAIHSLHTAHPVRRIAWRPDHPTELLVVPLTQPLSSSNSLDPALPASPPPPPPPPPALLSASSSTDNKLNMFTTTTTRDEPTMDDDSARLEIWHVRRHYIAKYAIPSQDGVAVDASWRGSSGLVVTFQNGGFAQLDIPRKLNSGLIPLPLDQIPRQIAGWSAKGDLYFAIDKFRAGEIPFDDLKPEYANHYERLGRPTHSLSDPPYIPLQTIGSLALPDPHTDPSELAFLANWYRLEGGIPADLCAWNRDVAKWCGREDDARLWGFVKGLFEEFMPQDRGGEEGTFVKDVWGRAGKPGARVITPPDVSPRGRHVRLPLDERLLPTYAGIPLERITPPASPPSNPSEISECSDVSDSGSSGEIYSDEDQSESSVKPRSKFISFIEPQSTSGLKLDLSAATIRKSSSFSSQEGSSPVFEKKTKKGQPGETSTSISTSPIAITNPLAGSVSHPNSISKKSNLSKIAFTPRQHQHQHQHQHHSHHPHQLVSSSDWPDPYGIIPDQVLTSGTMTGVASTTTASTTPTGTSRASTSQSSPVPPPAFGGRNMSNHNNNNNNDNSNNNNNAKNSPRAGMTGLAGHEIGSGVVEAPLAGRGSAEGGVGAVVVLPNGNGPGPGSRVGSGQGKEMRGREEKFEKKEWQEYRKKRVATLMTWWDGCVENGEMQLAATIALIASPLVNFPPFQVERLAHAYVQLLERHRLPIFAAYIRRFSGIPSLEITPQDEGLTHTYFCERCGKSTGTLEDIEVKGKVFWWCKKCRMGAKRCAVCRKTIKGLWMGCRQCGHGGHQACMRLYHSQAPLIPIPLANASASHASHIPHTLLNTSSSAYNQSAGSNFTTLSSTVEGTNTDLVEKVDGGEGAAESRFTLCPTGCGCQCRRIGAANTGHD | |||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| SEA2 | B14527 CNAG_04377 5’ flanking region primer L1 | TCGCAGAGTTGTAGGTACGC |
| B14528 CNAG_04377 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACTTGTGAAGCGATTCATCGTG | |
| B14529 CNAG_04377 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGAACGGGTTGTGGATGTCAAT | |
| B14530 CNAG_04377 3’ flanking region primer R2 | CCTGCTTCCTTTGGTCTCAC | |
| B14531 CNAG_04377 diagnostic screening primer SO, pairing with B79 | TCTGTCTGCCGTCCATGTTA | |
| B14532 CNAG_04377 Southern blot probe primer PO | CGGACGCTCCTGTCTCTCT | |
| B14883 CNAG_04377 Internal_LP | GGGAGGATGATGCGAGATTA | |
| B14884 CNAG_04377 Internal_RP | AGGATTCGTGATGGCGATAG | |
| B14885 CNAG_04377 diagnostic screening primer SO2, paring with JOHE12579 | AGTGGGACGAAAATGTCTGG | |
| B5501 NAT#123 STM primer | CTATCGACCAACCAACACAG | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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