Detailed Locus Information for CNAG_04411
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | hypothetical protein | |||||||||
| Gene Symbol | WDP6 | |||||||||
| NCBI Protein ID | XP_012051524.1 | |||||||||
| UniProt ID | J9VRP3 | |||||||||
| KEGG Linkout | N/A | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MSSTTDRIPNLIAHYIAIHYPAALDPFLQAAQIAAPDLSHPPNPDLRTVVEDWSSQQLATSLAMTTIDENNTDVPASDGTWKGWKLKDMMKAGFREGVGLRSTDRGFYGVSAANLLTVGAFHVPRREFDTTSAVYRTSCPLDIVTTSVDKTLKIIDYSSGEVNKALQPHRAAILTFAFHPQNPRYLLTGSMDGTTVLTDLLTYKTLQTFSSNKFVVRVLFSSDGHFMATASYDHHIVIYAATSSALPPPPNEDDLPLDDSDHRSLACEPDLQYTEVHRIQVDANPEAILFHPDSTWLMYTTRSSHQLYYVRLPSESCPPDVEWQIKTKSFNPHPMDTHVSFSVLNMALHPSGRMVACQTGDHRGGAGERILLYGVEPEETERLGCLWTGSDGDDYVLPRMAWVPDGSGIVTTTPNGFLSLIALNGEIRSSVKVHGTSNSGQAISEVVRDCCIIPGEAGGWEVISIGYDRSIRISVIDGY | |||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| WDP6 | B13963 CNAG_04411 5’ flanking region primer L1 | TTCGAAACCTGAGAACATCG |
| B13964 CNAG_04411 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACATGGCGATATAGTGGGCAAT | |
| B13965 CNAG_04411 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGCGAAGCATTCGCATTAGTGT | |
| B13966 CNAG_04411 3’ flanking region primer R2 | GCCACAAGCATCTTCAACAA | |
| B13967 CNAG_04411 diagnostic screening primer SO, pairing with B79 | AAGCAAAACGAAGACCAGGA | |
| B13968 CNAG_04411 Southern blot probe primer PO | CAGCGGAGGTTGTATCGAAT | |
| B14374 CNAG_04411 diagnostic screening primer SO2, paring with JOHE12579 | ACAAGGCAGAGAAGCGTGAT | |
| B14375 CNAG_04411 Internal_LP | TATGGATGGCACGTGAGTGT | |
| B14376 CNAG_04411 Internal_RP | GAGCCATGTTGAGCACAGAA | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG | |
| B6349 NAT#159 STM primer | ACGCACCAGACACACAACCAG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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