Detailed Locus Information for CNAG_04694
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | periodic tryptophan protein 1 | |||||||||
| Gene Symbol | PWP1 | |||||||||
| NCBI Protein ID | XP_012052151.1 | |||||||||
| UniProt ID | J9VY31 | |||||||||
| KEGG Linkout | N/A | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MSGTLISSLVWVPRGRAAERPKKYQLDEDEIERVGKLGGPGVLEQLKSEMAAVEDGEGMEGGDEEEDWEDENDEEINTKADNDDDDDEKMDEDKEPGKPFDPNDLSAFKMDEYDEEESKGVAMGAFANIKGLTYYRDNEEDPYITLKEDEEENEDEELALLPTDSMIISARTTSDLSSLDFHVYADVDENLYAHHDLMLPAFPLCVEWLDFSPGPDASGAAPEGAKPGSYVAVGSFDPSIEIWDADLVDGLYPRAILGQSPSLEKPEAKPLGTGKKKRKQMVQPAANADYHVQPVLSLSWTPNHRNLLLSGSADGTIKLWDLTRESPMGAMRSWDKIHGGEKVQAVDWNRSTVGGLDKVCLSAGYDRTVKVWDGRAVDEAIGVQVASDIECIRWDPWEPYSFYVSLENGLILSYDSRVLSSSKSSSRTTGSSKSSGFLTAAQPKFTLSAHDGPASALDINPHIRGCILTAGMDKTVKIWNVQDEESEGIPGRKREINLATSRDLGLGRVFAARWSPDPETPLTVAAAGSKATLQVWDVASNPGARKAFGERLRRHGRELGEIKKGGGIVAVDGGEEEESEGEE | |||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| PWP1 | B13606 CNAG_04694 5’ flanking region primer L1 | AAAGGTAGGGTCGAGGGAGA |
| B13607 CNAG_04694 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACTCAGTTGTTCGAGGACACCA | |
| B13608 CNAG_04694 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGGTAGCAAGGCAACTCTGCAA | |
| B13609 CNAG_04694 3’ flanking region primer R2 | AGTATCTTGGTGGGGCTGAG | |
| B13610 CNAG_04694 diagnostic screening primer SO, pairing with B79 | GCCTGCCCTCTCCTAGACTC | |
| B13611 CNAG_04694 Southern blot probe primer PO | TTACCAACGCCTTTGGATTC | |
| B14341 CNAG_04694 diagnostic screening primer SO2, paring with JOHE12579 | ATTACGGGGTGTACGGCATA | |
| B14342 CNAG_04694 Internal_LP | TGTCTCGCTGCTGACCATAC | |
| B14343 CNAG_04694 Internal_RP | GATTGGGTGTCCAGGAAAGA | |
| B5527 NAT#273 STM primer | GAGATCTTTCGGGAGGTCTGGATT | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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