Detailed Locus Information for CNAG_05084
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | phospholipase A-2-activating protein | |||||||||
| Gene Symbol | DOA1 | |||||||||
| NCBI Protein ID | XP_012048705.1 | |||||||||
| UniProt ID | J9VP10 | |||||||||
| KEGG Linkout | N/A | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MPKQYKLAFSLHGHAADVRNVTAPSQQVPLLLSASRDGSAIVWGPSNASREWDVKLRVEGPEKRYVSCVGMTRWGGQAFLLVGSSSGILASYVLPAMDSPAPTDDSSLPEPTHTLIEHSQNLCCMDVSQEGLIASGSWDKTVIVWKDFKKVIQIKAHEQAVWSVKFVGENRLLTASADKKIILHSVDPASGRATSLQTYTGHTEPVRGLALRSDRQGFWSCANDGNINIYSFDNPSPIRTLSGHTSFVYSIATFPDGSGAVTTGEDGTMRVWSETELVQTIPHTSNSLWSCAVVPSLVASSPYIVSSSSDSTIRFFTSEGALVAGPEELAAWDDEVKGRQLDKSQVGDVKHSDLPGIEALGREGKKEGQVLMIKNNGVVEAYQWSAPSSTWQQVGQVVDAIGQGRKQLYEGKEYDYVFDVDVSEGVPPLKLPYNVAENPWIAAQRFLERNELPTSYVEQVVEFIQKNTGGVQLGTGGDTASYTDPFTGGSRYTGGGVPITGAGGSSGSFGDPFTGNSRYTGGGISTTGNTTSSGDPFTGGSRYTGAATASFAPIQQSGAKGILPVKTYLPFKQINVSAAKNKIQQFNDELKTSKPELALTLEEEKTLTEVYAFLSLPAVALPDPNSQDGKEKFDTGAILALVQKWPEDKRFPLIDLARVLAATSPAFALSPPHAFFIAASLSLPFPDPPSKSRETNTLLALRAIANLFVTAKGRMTLSTQDVAKDILANVGGVEWGKVGKNVRIAGATVVLHLSILAVEGNLPAALGPPLLDLINQILDSEKEDTEVIYRSAIALGNLVSSPKAAGDLAVGKVAKGKESVKRWAGKESRLGSLATEIEALRL | |||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| DOA1 | B11073 CNAG_05084 5’ flanking region primer L1 | GCAACTGTCAGAAAAGACCTC |
| B11074 CNAG_05084 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACGAAGGCTTGAATCGGTTAG | |
| B11075 CNAG_05084 3’ flanking region primer R1 | ATGGTCATAGCTGTTTCCTGCTTGACTCCGAAAAGGAAG | |
| B11076 CNAG_05084 3’ flanking region primer R2 | GTGTGGAATCTGAAAACCC | |
| B11077 CNAG_05084 diagnostic screening primer SO, pairing with B79 | TCCTTCCTCTTCTCCTTTTC | |
| B11078 CNAG_05084 Southern blot probe primer PO | CAAAGAGGAATCATCTGTGG | |
| B12008 CNAG_05084 diagnostic screening primer SO2, paring with JOHE12579 | TTTCCAGCCCATACTCCATC | |
| B12009 CNAG_05084 Internal_LP | GTGCAAACGACGGGTAAGTT | |
| B12010 CNAG_05084 Internal_RP | TACAGCTGTTTGCGACCTTG | |
| B5497 NAT#102 STM primer | CCATAGCGATATCTACCCCAATCT | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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