Detailed Locus Information for CNAG_05219
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | hypothetical protein | |||||||||
| Gene Symbol | REC14 | |||||||||
| NCBI Protein ID | XP_012048543.1 | |||||||||
| UniProt ID | J9VPG3 | |||||||||
| KEGG Linkout | N/A | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MSLAYLEQDTTEALADSIWNISWNTANQLVTASADGHIRVWDIEELRQPVHDIDSHPLAITSLSVTDKKALASSLDGTIVLIDTENGEQLDKVESGRVKVSAEGPEIPAFACSLHPQSACWAWSGRSSKVVIRTMISDDADPTTQGPLGGESSIMDGGKGKFGMDLQFSPDGRSLALATDQGQVVVFDTETRATIATYSSHNKAVRTIGWSPDSQWLYSGSDDHLIVLYDVRAGSQSGAGGKGEGAVAMMQGHQNWVLRVAPSPDGRLLGSGGADHLIKLWDIGQRTCVSTSSSNADVWGFAWQPEGAGTLPPGKQFAVAGDDKVVTLYRAAGAV | |||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| REC14 | B14814 CNAG_05219 diagnostic screening primer SO2, paring with JOHE12579 | TATTCCGAGCGTCGCTAACT |
| B14815 CNAG_05219 Internal_LP | AGCTGAAGGACCAGGTGAGA | |
| B14816 CNAG_05219 Internal_RP | AGCTCGGACGTCATAGAGGA | |
| B17139 CNAG_05219 5’ flanking region primer L1 | CCGCCTAACCTACCACCATA | |
| B17140 CNAG_05219 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACTCAAAGTTCCAGAGGGTCGT | |
| B17141 CNAG_05219 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGATCTGGCAGCCACCTACAAG | |
| B17142 CNAG_05219 3’ flanking region primer R2 | TGGTCTTGGGTAGTTGTTGCT | |
| B17143 CNAG_05219 diagnostic screening primer SO, pairing with B79 | CATTGCGGACATACAGGTTG | |
| B17154 CNAG_05219 Southern blot probe primer PO | ATGTCCCGTAATCCTTGCAG | |
| B5531 NAT#296 STM primer | CGCCCGCCCTCACTATCCAC | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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