Detailed Locus Information for CNAG_05301
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
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| Gene Annotation | deoxyuridine 5'-triphosphate nucleotidohydrolase | |||||||||
| Gene Symbol | CRN1 | |||||||||
| NCBI Protein ID | XP_012048586.1 | |||||||||
| UniProt ID | J9VJD9 | |||||||||
| KEGG Linkout | KEGG::CNH03300  |
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| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MSRFVRPSKYRHVYGSKSKVNYENVKISGSAWDTDLVAAGGKYLSVNWQASGGGAFAILPLFSPTSPPQPAGFPTKLPDIVPLARGHTAPVLDTAWNPFDDNIVASAGEDGKIFIWKVEDSAFEGWGEDQWEPEDFNPALKLSAGGRKVGQVIFHPTSSNLLTAASGDHLVRLWDISSSADEPKLVLKGHGDSIQSIAWNSVGTTLATTCRDKKLRLFDPRAGSDAVRITDGHAGVKGSRVVWLGDRDRIATTGFSRMSDRQVSLWDTSSLKNLDTQSIDSSAGVLMPFYAEGNDVLFLAGKGDGNIRYYEFEGDQLHFLNEYKTSDPQRGMTLLPRRALDTQEHEIARAYKLSGGCVEPLSFIVPRKSESFQSDIFPPANSVEPAQTAADWFTGKNVRPNVVDLETGGVSASKEAVPTLTSSAPAAAPAQASAPSSTPSPAPAPVKEEPKSAPVYTPSPPAAPTQAPAPAAADVPIAVKKEIDSVPEITAKGLEIEQPEEASEDEQEEDKGPVEKVQDSLKSLTVGKEEPEKESNGVVAPSNTLLIKKLSPSATLPTRGSSLSAGYDLYSAEETVVPARGKALIDSGLCIAVPEGTYGRVAPRSGLASKHSIDTGAGVIDADYRGPVKVLLFNYSDSDFTIQKGDRIAQLILEKIVMAPIVEVEDLDATARGSGGFGSTGGFGKAVKDVAGSLI | |||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| CRN1 | B14248 CNAG_05301 5’ flanking region primer L1 | GCTGCCAAGCCATCTTTAAC |
| B14249 CNAG_05301 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACGTTAGTCCTTCCCCCAGCTC | |
| B14250 CNAG_05301 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGCGTCCATCGTGTAATGTTGC | |
| B14251 CNAG_05301 3’ flanking region primer R2 | ATTTGGCTGACTGTCGCTCT | |
| B14252 CNAG_05301 diagnostic screening primer SO, pairing with B79 | CTTTTTCTCCGGCACTTCAG | |
| B14253 CNAG_05301 Southern blot probe primer PO | TCCCTCAAACGCAGAGTCTT | |
| B15817 CNAG_05301 diagnostic screening primer SO2, paring with JOHE12579 | AGCCCTTGAGCTTCATCGTA | |
| B15818 CNAG_05301 Internal_LP | TCAATCAAAGCCTTGCCTCT | |
| B15819 CNAG_05301 Internal_RP | TCCCTTGGCCTGTACTTGTC | |
| B5495 NAT#43 STM primer | CCAGCTACCAATCACGCTAC | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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