Detailed Locus Information for CNAG_05465
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | guanine nucleotide-binding protein subunit beta-like protein | |||||||||
| Gene Symbol | GIB2 | |||||||||
| NCBI Protein ID | XP_012053792.1 | |||||||||
| UniProt ID | J9W3X8 | |||||||||
| KEGG Linkout | N/A | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MAEHLMFKGNLAGHNGWVTAIATSSENPDMILTASRDKTVIAWQLTREDNLYGFPKKILHGHNHFVSDVAISSDGQFALSSSWDHTLRLWDLNTGLTTKKFVGHTGDVLSVSFSADNRQIVSASRDRSIKLWNTLGECKFDIVEDGHTEWVSCVRFSPNPALPVIISAGWDKTVKVWELSNCKLKTTHHGHTGYLNTLAVSPDGSLAASGGKDGITMLWDLNEGKHLYSLDAGDVINALVFSPNRYWLCAATASSIKIFDLESKSLVDDLQPDFDGLSDKARKPECTSLAWSADGQTLFAGFSDNLVRVWAVVA | |||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| GIB2 | B20246 CNAG_05465 5’ flanking region primer L1 | GCGGCTACATGAGCTCTACC |
| B20247 CNAG_05465 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACGTTGCCCTTGAACATGAGGT | |
| B20248 CNAG_05465 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGCCGGTTTCTCTGACAACCTC | |
| B20249 CNAG_05465 3’ flanking region primer R2 | GCAGGACGAGATGAAGAGGA | |
| B20250 CNAG_05465 diagnostic screening primer SO, pairing with B79 | AAGGATCGCTTTGCGTGTAG | |
| B20251 CNAG_05465 Southern blot probe primer PO | CTAAGGGTGTGGTCCCAAGA | |
| B20342 CNAG_05465 Internal_LP | CTCTTGGGACCACACCCTTA | |
| B20343 CNAG_05465 Internal_RP | GTTGGTCTCATCGGGTCTGT | |
| B20344 CNAG_05465 diagnostic screening primer SO2, paring with JOHE12579 | ACGGTTTGTGAGCATTAGGG | |
| B5504 NAT#150 STM primer | ACATACACCCCCATCCCCCC | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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