Detailed Locus Information for CNAG_00528
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | WD-repeat protein 23 | |||||||||
| Gene Symbol | WDP5 | |||||||||
| NCBI Protein ID | XP_012046340.1 | |||||||||
| UniProt ID | J9VJ82 | |||||||||
| KEGG Linkout | N/A | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MPPREHYIVSSDDDEDGDYQPSDPEVALLDLLHDGYELYEEDYDEDDIDDEDEEDDDDLMVDENGYIIRDDGIGYDDDQEMSDDTESRAFADLLEFAQAVDDGESPLAFLSGRVSGSFPSILHRLAMRSDSRFRNNDWASKVKKKQTVANPKGTELLRSGEFGRVGNWQAPGKAGRQRSRGWQRAVKGWKPPRSTTSQHLVPNEPGTVVAYYPSVPYVGQFAGEDYSIFYTATQYFTLHLYSTTQYLKSKNNIHQRRTVRLSDSIRPTLTLSTPPARGNRVIEDEDQDGDEDDWEDEDDYVSGSSAIEDSSMKRIKRVQGVEGRWTITDCDADKKGEKMIYSSITPYVHMLYTDEFDQEHVELDFSDPRERGNYYRSGIWSIRFSADGKEIVAGASDGKIMVYDINAQRRSLSVSGHAEDVNAVCFADHSSTNVLISGSDDGYIKVWDRRSLSSHVPSGVLVGATEGITYTSPKGDGRYIVANSKDQAARLYDLRKMRSYGDFVDEPNASQKYGAAGFDYRDMRYPRSEPRSHPQDCSVMTYSGHSVLRTLIRCHFSPIESTGQSYIYSGSADGMIHVWSLDGRVIQVLDRSASEGLYSSQGIYSDPSAAPRSPQSSRTNLFGSYSHAVRDVAWHGYEPTLMSTCWDMMGSMRRGGTIAKHEWKGLGKNGLAKLEDWEMKRKEENEGSRATS | |||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| WDP5 | B13755 CNAG_00528 5’ flanking region primer L1 | ATGCATGTCATCCAGCTCAC |
| B13756 CNAG_00528 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACACCCATCATGAAGGAGATCG | |
| B13757 CNAG_00528 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGTGGGAAATGAAAAGGAAGGA | |
| B13758 CNAG_00528 3’ flanking region primer R2 | TGTAATTCGTTGGCCATGAG | |
| B13759 CNAG_00528 diagnostic screening primer SO, pairing with B79 | TGGGGAGCATAGTGCAATAAC | |
| B13760 CNAG_00528 Southern blot probe primer PO | TCACTTCTCATTGCCAGTCG | |
| B22985 CNAG_00528 Internal_LP | ACGGAGACGAAGATGATTGG | |
| B22986 CNAG_00528 Internal_RP | TCATCGGAACCGGAGATAAG | |
| B22987 CNAG_00528 diagnostic screening primer SO2, paring with JOHE12579 | TGACCCACAAAAGAATGCTG | |
| B5522 NAT#230 STM primer | ATGTAGGTAGGGTGATAGGT | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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