Detailed Locus Information for CNAG_05795
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | mitogen-activated protein kinase organizer 1 | |||||||||
| Gene Symbol | WDP11 | |||||||||
| NCBI Protein ID | XP_012050535.1 | |||||||||
| UniProt ID | J9VU23 | |||||||||
| KEGG Linkout | N/A | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MSVAKVAASKGPSDEKAPSILSYPTKLLQTLDAHQAPVNVVKYNNGAKYLLSGSADRTIRLWNPALGKEIKCYRGHAQEVLALDIAQDNAKFASSGGDKAVFVWDVASGAVTRRLQGHFGKINAVRFSQEAQVLASAGFDAKVMLWDMRASSRDPIQTLKEATSTITSLILPTSPQIITGSSDGYIRTYDLRFGLLTDDLVGWPITSLKLSPTAPEESVLVGTNDGKVRIFDRKDGGCLQTFQGHKVREQTGRWGMDWGYGDGLALAGDEEGKLWAWNVLDAKPLDKDPNPIHKRTITSIITHPKGKEMITASNDGTIKVWTR | |||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| WDP11 | B18256 CNAG_05795 5’ flanking region primer L1 | TGAGATGGATAGGAAGGATAAGC |
| B18257 CNAG_05795 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACTGCTGATGGTGAGCAAAGAC | |
| B18258 CNAG_05795 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGCTTGACAAGGACCCAAATCC | |
| B18259 CNAG_05795 3’ flanking region primer R2 | TTATGAAGCCCCGGTTGTAG | |
| B18260 CNAG_05795 diagnostic screening primer SO, pairing with B79 | AGAGGAAGCGTACTGCCAAA | |
| B18261 CNAG_05795 Southern blot probe primer PO | ACTGCCTTGTCTCCTCCAGA | |
| B18493 CNAG_05795 Internal_LP | TCTGGAGGAGACAAGGCAGT | |
| B18494 CNAG_05795 Internal_RP | GACGTTCCATGCCCATAACT | |
| B18495 CNAG_05795 diagnostic screening primer SO2, paring with JOHE12579 | AGGCTTCTTCGACCCTCTTC | |
| B5531 NAT#296 STM primer | CGCCCGCCCTCACTATCCAC | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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