Detailed Locus Information for CNAG_05816
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | hypothetical protein | |||||||||
| Gene Symbol | WDP4 | |||||||||
| NCBI Protein ID | XP_012050552.1 | |||||||||
| UniProt ID | J9VWP8 | |||||||||
| KEGG Linkout | N/A | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MPAPPPIAYATLTAPLPAAPYILGITPSPTTPHLVLRHPSQSLTIADNQTLQPVAALQGGHAEHISSVSCDGGELWSAAVDGSIVRWDERGRQKATVIKAFIRKPLPVTALATSANDNLVIGGTELVSSEAHILFWDPRNSSAPLYTHSSTHSDDITHLSLLRSSSTFLPPSHNSEIKTPSTLLLSASTDGLVALSDMKESDEEEALVAEENWGQSIADAGGYMHKGKMSVWARSDMDEMCLWGVGRGNIGEIELQDHKHYASSEFKFKTFTPPKAGPSITQTALDEFKSKTTYKSDYLINVVPSLGVSSDGAPMTTVGTNEGDMILQHHSASTSSYKPSAFFLSGPGANRGHKDVVRAMYHDLANEALYTGSEDGVLAGFSLASLPERLTVGDPEVDDDDGDGREEDDNMDEESEIETESSEESDDDEDEDMEEEGPRYGPIIGAGSNGRPGADKKEERRKKRYGPY | |||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| WDP4 | B13683 CNAG_05816 5’ flanking region primer L1 | ACGTACGTGCACTGTTTTGG |
| B13684 CNAG_05816 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACGCTGTAAGCGTGGCGTATG | |
| B13685 CNAG_05816 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGTGGCCCTATTATTGGAGCAG | |
| B13686 CNAG_05816 3’ flanking region primer R2 | ACGGCCTGTGACAGAAGAAC | |
| B13687 CNAG_05816 diagnostic screening primer SO, pairing with B79 | GCCTGGTCCGATATCACTTC | |
| B13688 CNAG_05816 Southern blot probe primer PO | CGAGCTGATGTGTTCTGCAT | |
| B14419 CNAG_05816 Internal_LP | TGACATGAAGGAGAGCGATG | |
| B14420 CNAG_05816 Internal_RP | GCACCTGGTCCAGAAAGAAA | |
| B14421 CNAG_05816 diagnostic screening primer SO2, paring with JOHE12579 | CTGGTTGTTTCGCGGTTTAT | |
| B5520 NAT#220 STM primer | CAGATCTCGAACGATACCCA | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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