Detailed Locus Information for CNAG_05824
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | WD-repeat protein 68 | |||||||||
| Gene Symbol | DCA7 | |||||||||
| NCBI Protein ID | XP_012050559.1 | |||||||||
| UniProt ID | J9VPG2 | |||||||||
| KEGG Linkout | N/A | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MSPYPLIDYDVGQPLYGVGFSNSVSHPYRVALTSLVTGPTNKLYIADLSHPPDTQPSSPHYPQANNGSSGPPHPPPTYRQLTSINLNLPATKVGWEPEGSVRVDQGGRGELVATTGESLHLWEVATGWTEGSGHVGHNGWGNERYALKSRSILSNTKGAHGSLPPVTSFSWNNKSPNKIVTCSIDTTATLWDINTAQAMTQLIAHDRAVYDLCWLPESSDIFVSVGADGSLRAFDLRQLEHSTILYESSRDAPLARIAFSKKEQHMLACFGLDDSKILILDMRSPGKPVAELIGHQAPLGAIAWGSGGTRGRGEPTGGGWLASCGDDSQLLLYDLTAPLPTSRSSSRSNFQNSNPTSPYVLSPSATPSSQRTPSPADAVEMMPVKGWTARGEINNLAFTNDGDWVGCVSGTTLKVLHV | |||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| DCA7 | B11089 CNAG_05824 5’ flanking region primer L2 | GCTCACTGGCCGTCGTTTTACGTGGATAGTGTGGAGATGATG |
| B11090 CNAG_05824 5’ flanking region primer L1 | TCACCACTTGTCACTATCTCC | |
| B11091 CNAG_05824 diagnostic screening primer SO, pairing with B79 | TTCAGGCTCAAGACTGCTAC | |
| B11092 CNAG_05824 Southern blot probe primer PO | CGCTACTTCCCATAAATGC | |
| B11093 CNAG_05824 3’ flanking region primer R1 | TGGTCATAGCTGTTTCCTGCTTCGCCCTATGTTCTTTC | |
| B11094 CNAG_05824 3’ flanking region primer R2 | ACGAACATTCTCAACAGAGG | |
| B12035 CNAG_05824 diagnostic screening primer SO2, paring with JOHE12579 | ACAAGGGAAGATGATGAGC | |
| B12036 CNAG_05824 Internal_LP | CGTAGGTCGCATAGCCTCTC | |
| B12037 CNAG_05824 Internal_RP | GCATGTCGAGGATGAGGATT | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG | |
| B6349 NAT#159 STM primer | ACGCACCAGACACACAACCAG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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