Detailed Locus Information for CNAG_06282
| Species | Cryptococcus neoformans var. grubii H99 | ||||||||||||||||||
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| Gene Annotation | WD-repeat protein 48 | ||||||||||||||||||
| Gene Symbol | BUN107 | ||||||||||||||||||
| NCBI Protein ID | XP_012053378.1 | ||||||||||||||||||
| UniProt ID | J9VVN3 | ||||||||||||||||||
| KEGG Linkout | N/A | ||||||||||||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | ||||||||||||||||||
| Functional Annotation |
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| Amino Acid Sequence | MAFQLPQPVIPGRRVSIFLQGPSPGDPPPTLSLPPLTIDRRHHPALYIIPKTPSSSGVTLGSRNPFLKTNNNPSPKKKEDDHPRHASGVNALALDTTTVLKGESNPGGILYTAGRDGMVASWDLGIPHTRRTSRRYSPIDGRKLRKVQWEFIGDGADFFRANEEDGEGGSDVEGEGDGEEGWVDMKETMNEIPFEDRYEIDKAELSKRSPKTTFRQSSQTHTDWVNSLLLCNYNRTVITASSDRTIRAWTPHSSLSASSPSGAEDPSRPSPALVGTHTDFVKSLAYAEGPGYLWSGSLDQKICLWDIQQGPGDCQPILDVDLQAEGVYGGGVYTLAIDNTGSTLAAGTTERIVRLYDARAGPRSTGHLVGHEDRVRSMLFSDDGKYMLSASSDSTIKLWSLAAHRCVHTFTHHDSPVWALHSNHPNFERFYSGSRDGRLCVVDVEGCRDLSGGEAVLLAGSERETQYGERGIRSIVAMDDEYVWTSSGMEDVVRWKDVGRRVGRLDKVFDGASYRSPVQAEGGGMEREPGRVAFAPSPRDPLLGGEASVSALSANVRDRLAVGHRSSLSNSSFVSSSASTIAGDDGRRDEDQEQGEGGVNTSLTRNGIAYESLINLSVDRGLYGYEYEESAISVSGDYTSSAPQAAQRLRPSALYAQRDLACLARPIQPHPPLDDPGIIRGRTGLIRSEPLNDRIHVLTADADGQVALWNIVKGICVGVFDPKELCEALHISPTPSAEASRAFPRTGPPLEPEVEAVRIELRRHSQDVLNMIREHVQGMTSVSQWFSSDTKTGSLAVHIEERTAFVGDVYLNDIGLKVEGGEGGEDQKINLGQWTLANLFRGFIAAEEHEVVSHLPNSPSTLSSSLPSTHGVPNSHDNSSRLRSPTLTTSIDRSPISPGHRKRALTGSFSGTRPPAASLVIPGLVSPAARPAVELDMPTDVFGSGSVEGKGSLLRSISCRAVGTPGGFSIGSMTSPGLSGEGISIASSVPNGNGGRDYFSPRKKTEQTIGGALASSGPGNGDREGSSPATKGQGTPSTPGGSFMGRIKIKNFGKKKAAEASMPAVQERAATPEDTGPKRSERDIQQLEMLDNIRAHHFHPPPPWEAPYVAYPPSTTLLISEVWQDTGAWVVTYRSEVGSTERDMEPLETNSPGWLLWYLFTNTIPPKPQHLVNIRFKLEPFPGSDLPELPKGESQVNAPKTLRCNKVYQYVAERLNAINEAQNHRSKKRKLTAEDIDLVIMTDPEIGSLDYRMTVGTVKAYYAPRGDYGFWYSLKQKK | ||||||||||||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| BUN107 | B14091 CNAG_06282 5’ flanking region primer L1 | CGATTGAGCTTGCATGAGAA |
| B14092 CNAG_06282 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACAGGAATGGGTTCCTTGATCC | |
| B14093 CNAG_06282 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGGATAAATGAGGCGCAGAACC | |
| B14094 CNAG_06282 3’ flanking region primer R2 | CGAGCTTCGTGGTCTTCTTT | |
| B14095 CNAG_06282 diagnostic screening primer SO, pairing with B79 | TTGGTCACAAGTCCGTGAAA | |
| B14096 CNAG_06282 Southern blot probe primer PO | GAATGTCGTTTTGGGAGACC | |
| B15004 CNAG_06282 diagnostic screening primer SO2, paring with JOHE12579 | CCCTACCCTCCTCGTTCTTC | |
| B15005 CNAG_06282 Internal_LP | TCTCCGTCTCTGGCGACTAT | |
| B15006 CNAG_06282 Internal_RP | ACCTTCTCCACCCTCCACTT | |
| B5507 NAT#177 STM primer | CACCAACTCCCCATCTCCAT | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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