Detailed Locus Information for CNAG_06382
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | beta-transducin repeat containing protein | |||||||||
| Gene Symbol | WDP10 | |||||||||
| NCBI Protein ID | XP_012053455.1 | |||||||||
| UniProt ID | J9W363 | |||||||||
| KEGG Linkout | N/A | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MLSIKGQSSLSSEDLSSEPPSALPPRLKSKQNVPPSWSPNTTASNRRRLGGLFADLGVPTSGSPLKATPARVEPTPTKSGRKSGMMETLSSPITSFADRLAVLSMVASGEVHQSESPVKQKSKSKQVKDGKEHLTDNEGVACERKRDIEEGLKLEHLPDDLILEVLLQLPPTPQALSPVSSLSKHFYNLSRAPILWARIFSAAGYTSQLSQEVLERGLGVWEGPKGQWDGLTWVAETQGTIDEIEEEVPSEYIPVHYPTLHRTACTLPQHIRSLLSAHRASFSTLSGHTESVYCVQSVGNWLITGSRDRSIKIWMLPLVNSDEEARLVITIPNAHNGSILGLFFELDDKGRGLLVTSSSDCTASIWSLDLSPYSQRKSVAVSKLQTLLHPLAVLDVALTPSSIVTASKDCHVRVYSRNSFELVHLLAGHRGPVNCVTPRKVDWISREKGEQREEVVSASGDGSWIVWDIKNGCQLRKGADVGRGLACVAWEDDYILTGDNECLVKLYNAETCKLLKVFQGHSNLVRAVALRVRDGMAISGSYDESVMIWDLHTGHLIKRPTLEHHSLIFDLEMSCKRLILVGHGHSVQVLTWGKGLPYVDFLV | |||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| WDP10 | B13713 CNAG_06382 5’ flanking region primer L1 | TGTCGACACATTCTTGGGTTA |
| B13714 CNAG_06382 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACGGAAGGTGGTTCTGACGAAA | |
| B13715 CNAG_06382 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGGGCAAAGGCCTTCCTTATGT | |
| B13716 CNAG_06382 3’ flanking region primer R2 | CGCAGACAAAGGATGTTTGA | |
| B13717 CNAG_06382 diagnostic screening primer SO, pairing with B79 | CCTTCGTGGTGTTACCTCGT | |
| B13718 CNAG_06382 Southern blot probe primer PO | AGGAGATTCGCTCTGATGGA | |
| B14347 CNAG_06382 diagnostic screening primer SO2, paring with JOHE12579 | TCACTCGGTGCAATAAGCAG | |
| B14348 CNAG_06382 Internal_LP | GAGGGCTCTTTGCTGATTTG | |
| B14349 CNAG_06382 Internal_RP | CCCATACCCCTAAACCTCGT | |
| B5526 NAT#242 STM primer | GTAGCGATAGGGGTGTCGCTTTAG | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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