Detailed Locus Information for CNAG_06597
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | transcriptional activator SPT8 | |||||||||
| Gene Symbol | SPT8 | |||||||||
| NCBI Protein ID | XP_012050721.1 | |||||||||
| UniProt ID | J9VN65 | |||||||||
| KEGG Linkout | N/A | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
|
|||||||||
| Amino Acid Sequence | MRSEEEDDDDEYYDEHEEEDDERASNGDNGEAEGDEDDADEAIEEASDEEGSDEDNAEEDAEVEDEEEEEEEEDGEAEEDEEDERDEEEDGEEASEDEADGEDVTMAAAQDDEAGSIHPSQVSKSAKLSRADLPPPPHLIRRSLFKPAFMAPPTTLSVEAIVGIPLPTPVQSLASSTCLSYLLTGGQDGFVRAYDFWGSVNGSQMMTAQQRSVVGLGEGMNKAGLPRGWWTNEVEGIIGGTVAKRTEPVYSMACEGDALWALTGTQSGPINLSSLRHSPGHLVHTLKGHTNVVSCMTLLPEEKGFISGSWDGTVREWDLNTGQTVRTYPTHKAQLSSVSLRPVGFPSSPTPSPRPKAPGEEEEEEEGNEDPAMNISISMGPDFFGKKEMGNLKQEGENGKESSGDKEAEILSEQKPSNDIEMADAASAVSGTGDSLFGDDDAEGETAPASILPTTIPSPSLPSPPKPKPLGLALPGQRQSSSAPISTKETEAAPVSAPLFAPQASASSARQGAADVIPVLSPVNWKAYSEDVLLTSSMDGQLVLIDRRVPSYEGTGAGVGRLLPGEKTPPWCMSACWLSNGNQVLAGRRNGTVEIWDVRKGSSSTGPNLLRSLKTPVESGPISCVVAFPDGRHIATASQDNIRLWNAAEVFHSEDSAKRSKGKTPFKIIAGHHGGTISSMIVDRTCRFLITASGDRGWGGESTKAVLIHEVKW | |||||||||
| AlphaFold Structure with WD40 domains |
||||||||||
Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| SPT8 | B14425 CNAG_06597 Internal_LP | GATGGTGGACGAACGAAGTT |
| B14426 CNAG_06597 Internal_RP | GGGTCTTCGTTCCCTTCTTC | |
| B14427 CNAG_06597 diagnostic screening primer SO2, paring with JOHE12579 | ACTGACCTCGAGTCCGAGAA | |
| B14657 CNAG_06597 5’ flanking region primer L1 | GGGCCTAAGACCCCAAGAGAC | |
| B14658 CNAG_06597 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACGGAGAGCGTTTCTGGAGTTG | |
| B14659 CNAG_06597 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGGCTGACACCCAGTTATGCAA | |
| B14660 CNAG_06597 3’ flanking region primer R2 | CCATCTCGCTTCATCGTTCT | |
| B14661 CNAG_06597 diagnostic screening primer SO, pairing with B79 | TCCCTCGGGTCTTCAATATG | |
| B14662 CNAG_06597 Southern blot probe primer PO | TACCTCATCCCCAGACCAAA | |
| B5518 NAT#218 STM primer | CTCCACATCCATCGCTCCAA | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
|---|---|
 |
|
| Mating Data | |
 |
|
| Virulence Factor Production | |
|
Capsule
Urease
Melanin
|
|
| Stress Response and Adaptation | |
|
Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
|
|
| Antifungal Drug Susceptibility | |
 |
|
| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
 |