Detailed Locus Information for CNAG_06603
| Species | Cryptococcus neoformans var. grubii H99 | ||||||||||||||||||
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| Gene Annotation | di- and tripeptidase | ||||||||||||||||||
| Gene Symbol | DUG2 | ||||||||||||||||||
| NCBI Protein ID | XP_012050288.1 | ||||||||||||||||||
| UniProt ID | J9VNU2 | ||||||||||||||||||
| KEGG Linkout | N/A | ||||||||||||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | ||||||||||||||||||
| Functional Annotation |
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| Amino Acid Sequence | MAPSSPILSPSRSPLSGGRVNGDNASVNERTALQSGMLGERKDSGVRKDSLTREKKGKMPVLSHLLNVGNGTVLSLAADEKYVYAGCQSADNEITVFSRSSLQPLYRLLGHQGSVLALLIVEEKKWLVSSSSAGDVRIWSTETLDLLYVIHPCDDTSGDIYSLAWDERAGGTLYFGSQSCSIEWINFSDLCTFHQRKISGSAAGTVHVVPNGIDSSSRPGPSQRSGRYKPHKFFDNPPENGCSGSSTPCSPFPSNTARKETFSKAELDQVSVNLLEQTRPATEIEVPAESRLAFAHYGYVYALHTLSRPNGDKWLVSGSGDSDVKIWECHPSGGITLIRQFNSLSGAVLSFAFRDDLLYAGLQAGEIAVWDLETGACIRTIEAHEEDVLVMSALGGDVYTAAADGRVLRVNDEFDCTAAFKAHSGTVMSSTIVKSVKGDSWELITGGNDSYVKIWQVEASKETLQSGNVMDMEHEGDVMLYALSKLVAVPTISDDSHRESCRQGAHLLKKILSQLGASSEVLCGEQGKNPLVLATFTGRDIGKPRKRLLFYGHYDVQPAAEKRWITNPWELSGRNGYLYGRGVTDNKGPIMAVACAAASLRQRRELDVDLVMIIEGEEEAGSRGFASTVRAHKVDIGHIDAILLSNSTWIDEEDPCVVFGMRGVVYANLSVESNEENLHNGVDGGASSEPMFDMVRVLAALSDAKGVKVPGFYDSVRPATNEEMSLLRDVSSACGRPLDELIRVWRQPSFSIASINSSGSGNKTVIPRKVSTDISMRIVPDQDLETIVKGLKQFCRDTFQGLGSPNTFKIQVTHTASWWLASLESPYFKALEASVQDVWGVKPLKIREGGTVPTVFWLEKEFGAPCVHLPLGQSSDAGHLANERMRLLNLRNGKRVIEAYLTRLASI | ||||||||||||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| DUG2 | B13519 CNAG_06603 3’ flanking region primer R2 | CACTCCAAGTCCGTGTTTCA |
| B13520 CNAG_06603 diagnostic screening primer SO, pairing with B79 | GTCAGTGGTGGCAATCCTTT | |
| B13521 CNAG_06603 Southern blot probe primer PO | ACCCTTCCTGACTCCGCTAT | |
| B13678 CNAG_06603 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACGGATGGCGATAAGATTGGTG | |
| B13679 CNAG_06603 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGCACGAGACTCGCAAGCATT | |
| B16518 CNAG_06603 5’ flanking region primer L1 | TGCGAACCACTTGGATGTAA | |
| B17541 CNAG_06603 Internal_LP | GTCTTGGCAGCATTGTCAGA | |
| B17542 CNAG_06603 Internal_RP | ACCGTTCCTACATCCAGTGC | |
| B17568 CNAG_06603 diagnostic screening primer SO2, paring with JOHE12579 | TGGGTGATGTGCAGACTGTT | |
| B5519 NAT#219 STM primer | CCCTAAAACCCTACAGCAAT | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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