Detailed Locus Information for CNAG_06733
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | hypothetical protein | |||||||||
| Gene Symbol | RPN14 | |||||||||
| NCBI Protein ID | XP_012047697.1 | |||||||||
| UniProt ID | J9VI49 | |||||||||
| KEGG Linkout | N/A | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MGGSNQVENMSYVDIQHDAIDVFDDVEQAIVMKEDIWISGYQQGETSVHGKAMVEFEDGGSISIIARDGVNVERVTKTDFVVDIPALGISKRPVHFPKHILHPPYKKISDVDPPLQINALAVNPKTPHIVIGGPDGYCMILPTGVMASPEKDGVKLKGHVGDIRAVKWFPSGEVVLTASADLTIRVFGMNGVNPRTFKGHSRAITSLVILGVGKTFLSAAKDGTIRHWDVGSGQQLKQWTTGGEKGTLVIEAFILVEESTAIEALGLQGQERVMLVQDQLAVWVQPWDGEGWLVEADKQIAFLAYERETGTIAIGYSSGIVEIRDIASLIPGTIGKRKKIARNESPIYSLAFGLDVKDGGMDLYLGTAAGLPCRLGVKCVADGYQVVMKDELAGWDAVGIECMEAVRDGMWCGGGEGGLRRYTV | |||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| RPN14 | B17024 CNAG_06733 5’ flanking region primer L1 | GGAAGAGATGGCGGAATGTA |
| B17025 CNAG_06733 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACCGAATATGGCTCACCTGGAT | |
| B17026 CNAG_06733 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGGTGGCAGACGGATACCAAGT | |
| B17027 CNAG_06733 3’ flanking region primer R2 | TCGAGGCGTTAAGTGAGTTG | |
| B17028 CNAG_06733 diagnostic screening primer SO, pairing with B79 | ATTGGCAGGAAGTGGTTGAC | |
| B17029 CNAG_06733 Southern blot probe primer PO | AGCCCAGATTCATGTCTCGT | |
| B17124 CNAG_06733 Internal_LP | GACCCAAACAGCCTCCATTA | |
| B17125 CNAG_06733 Internal_RP | AATTTCCACGATACCGCTTG | |
| B17126 CNAG_06733 diagnostic screening primer SO2, paring with JOHE12579 | ATTGCGAGCTTGTTGATCCT | |
| B5526 NAT#242 STM primer | GTAGCGATAGGGGTGTCGCTTTAG | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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