Detailed Locus Information for CNAG_06778
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | serine-threonine kinase receptor-associated protein | |||||||||
| Gene Symbol | WDP1 | |||||||||
| NCBI Protein ID | XP_012047728.1 | |||||||||
| UniProt ID | J9VFA5 | |||||||||
| KEGG Linkout | N/A | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MQGYSNGYSGFPASLNPNPAVGQYAPPQQAQLSIQSRSGSVPSSQSHEAIKVQPLLCSGHTRPVTHLQFSNVLDDGTYLLISACKDGNPMLRSWLGDWIGTFIGHKGAVWSSKISLDTSRAVTGSADFTAKIWDTYTGEALHTFSHNHIVRTVALNPQQTPQYLLTGGHEKKIRLFDLGRPDAEPLVLGTNPDGLSCEGIVKSLVWDEGQNGTMGVSAAEDGKVRWWDLRTLSQVASLDLGEPISSMELAHGGGTLSVTAGKNVHFLDILRQHPPVTIPLPHPVTSASLHPYLRDRFVAGSTSDPWVRVYDLDSGKEREVYKGHHGPVLCASYSPDGEVYASGSEDGTIRLWQTNPGKAYGLWQTSD | |||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| WDP1 | B16260 CNAG_06778 Internal_LP | GGTAGGGTGGACACGAGAAA |
| B16261CNAG_06778 Internal_RP | ACTGTAGCTCGCACAGAGCA | |
| B16262 CNAG_06778 diagnostic screening primer SO2, paring with JOHE12579 | GCAAAGGATGGATGGAGAAA | |
| B18268 CNAG_06778 5’ flanking region primer L1 | CGTGTCTGTCTCCACTCGAA | |
| B18269 CNAG_06778 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACTAACCATCCATCACGCACAC | |
| B18270 CNAG_06778 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGGGCAAGGAGCGAGAAGTGTA | |
| B18271 CNAG_06778 3’ flanking region primer R2 | AGAGCAAAGAGGGTCCAAGG | |
| B18272 CNAG_06778 diagnostic screening primer SO, pairing with B79 | CTGGGTTCCACGTTTAGCAT | |
| B18273 CNAG_06778 Southern blot probe primer PO | AGGGTTCAAAGCAACTGTGC | |
| B5512 NAT#205 STM primer | TATCCCCCTCTCCGCTCTCTAGCA | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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