Detailed Locus Information for CNAG_06888
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | cytoplasmic protein | |||||||||
| Gene Symbol | SEA4 | |||||||||
| NCBI Protein ID | XP_012047998.1 | |||||||||
| UniProt ID | J9VNG1 | |||||||||
| KEGG Linkout | N/A | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MATECRRIALADPHHLAPPRFLVGGQPAHMLSGDVKLYEWDRDRGKMSMLGLQTEIGQIKALAWSPSPAHRHLVATGLSTGKTLLLNLSPSTLSLPVAGPPPSAPVIAALTVKHTRAVTSISFSPHDANYLATGLERHRSDSSLLIWDIHDAVAASRLPPDGDVHYTRPELRLPTTTPLTKTSSASEPRPIQAYCASEQVNSIAFIPTAPYSLLASAGNKAIRLYDLRAPSNTLKEPNSAGSLAQWSTRTVMSLCPNPSSALFASYESNQGGGNSTVRLWDTRYPGQEVVGWEVRGGVVGMSWVDSMRLGVGSKEGGVGVWDIVRCKPDQSGASEWVTLGGMRQIIKPKPNMHSFAFTPPTPPKHVDVMYVLKDGTISIGPISTAPVLASNSHGGVSISEPALYFIDPDIPLRSSSSSTSTSASTSPAAHGLMQGLEPGQGEAGRGGGGGGEAIYPRNKFQLPPDQVSTILNEHSRLRSSSFGAASTIHPSSSPLHPHPHPHPHPPPTRHLPSGSGPNVAIHHIPDTLTQSFLSRDRWHEDDPTGVINDRDEITGGYEGWRRVLGGDVGVVMRRRAMEGYGLDNLLLNAAIATKYPGKFKLAGVWEFVEHLTKTMSPSVSSSGGYNLTHQGIYPIWTSLGTLDAASASGRPISAALNVDEDGAVGEGGGLVSELEERLKSMSIKGGGASTRRGSRSASVSRKGSVSRKGSGTHTPRERKVSERTTHSHSHPHSHPHTSSTYPQGPPSYLPAISHLLSSRLSFPDKAIHPSELRPSISSSSEKVELRRLILTICGESKEGGKGEVEEMLRRGERSKAAFRAFFRGDESGTVGILMSSEDPNDTLLGSTIAGFMSQSASTRGSEYFNAHWPNLIRRVDDPYVRAILSRIAGEDWESVLEEEYIPLLERMVVGVQYLDDWEFSTFLKGRMSRFARSSSLHMLPLTGLSPPALSLLSRYLARTGDLQTVTLLAAFFPPGKLSQAEKMMVERWREGYRDMLDSWGMWGERCEFDVKWGNLQRSLGGDDAGEEGDGKMGTCPVCNNPLSVDTEHRLHQKHAMRGTPSVGWPSERTTVCVYCQAPLPRCVICLLHVDPHRPPVDDGERPGHVTDTIDSAYVFCLTCRHGGHANHILPWFEGGLDGGIAHSVCAVADCDCECANI | |||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| SEA4 | B18603 CNAG_06888 5’ flanking region primer L1 | GCAAACGTTGTGGAGCCTAC |
| B18604 CNAG_06888 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACATCTGGCCGATCTCTGTCTG | |
| B18605 CNAG_06888 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGATGCATTCATTCGCATTCAC | |
| B18606 CNAG_06888 3’ flanking region primer R2 | CCCGTCAACTCACGTTATCC | |
| B18607 CNAG_06888 diagnostic screening primer SO, pairing with B79 | GGAACAATGACAGAGCGTTG | |
| B18608 CNAG_06888 Southern blot probe primer PO | TCAGAAGCGGAGGATGTCTT | |
| B20285 CNAG_06888 Internal_LP | CCGTGATCAGAAGGCAAAAT | |
| B20286 CNAG_06888 Internal_RP | AGATGGGGTAAATCCCTTGG | |
| B20287 CNAG_06888 diagnostic screening primer SO2, paring with JOHE12579 | GAGGATTGTAGGGGTGACGA | |
| B5506 NAT#169 STM primer | ACATCTATATCACTATCCCGAACC | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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