Detailed Locus Information for CNAG_00567
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | WD40 repeat protein | |||||||||
| Gene Symbol | RRT2 | |||||||||
| NCBI Protein ID | XP_012046809.1 | |||||||||
| UniProt ID | J9VF36 | |||||||||
| KEGG Linkout | N/A | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MSLSRAKSLANVDTLYSADSIEFCPFEGFQDLLVCGTYQIVEPENGGRTVDADVSDDEQAPKQTQRVGRLLLFQVGHGHHLQELQKIETSAILDIKWSPNPAGGPQLAVADAKGHITVFGLDVEIKRLREMQRLDVADETKLCLSLDFSNRRNLMAPSEIITSVSNGSLAHLIPASSGYVLSSSWPAHDFEPWITTFSIDDPKTVWSGGDDCKLKRWDLREPGRPTFVNKNFEAGVTTITPSPHTPNLLAVGSYDENLRFFDARSAMEPLLTIPMGGGIWRTRFHPHAERAGDLLVACMHDGFKIVHVSQGMTGGDWESDYNVNGSVVKIFEEHSSLAYGADWSRLPMEEGESLVGTCSFYDHTMHLWQG | |||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| RRT2 | B13761 CNAG_00567 5’ flanking region primer L1 | GGCTTCAAGGGAAATGTTGA |
| B13762 CNAG_00567 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACAGGGACATGACGCAGTGTAA | |
| B13763 CNAG_00567 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGTGTGGGCACATGTTCATTCT | |
| B13764 CNAG_00567 3’ flanking region primer R2 | AGATCAGCGCAGTCAAGGTT | |
| B13765 CNAG_00567 diagnostic screening primer SO, pairing with B79 | AGCTTGCGCAATTTGAGTCT | |
| B13766 CNAG_00567 Southern blot probe primer PO | CACCATTTTCTGGCTCCACT | |
| B14314 CNAG_00567 diagnostic screening primer SO2, paring with JOHE12579 | TGATTCTGCCGATCTCACTG | |
| B14315 CNAG_00567 Internal_LP | CAGTTGCAGATGCAAAAGGA | |
| B14316 CNAG_00567 Internal_RP | ATCCAAGGCTCAAAATCGTG | |
| B5499 NAT#119 STM primer | CTCCCCACATAAAGAGAGCTAAAC | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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