Detailed Locus Information for CNAG_07548
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | cytoplasmic protein | |||||||||
| Gene Symbol | WDP7 | |||||||||
| NCBI Protein ID | XP_012048401.1 | |||||||||
| UniProt ID | J9VIN5 | |||||||||
| KEGG Linkout | N/A | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MQTSDPGHLFQTTAQLKQAASRERKLKAAEKIGSPITLSSKVLALEIRDSHAFTAESGWQARRVDLKTGKTIRLYRGHQGPVTSVALMRIQGEKPTDILLTGSWDKTVRVWDTDTGAHLQTIEGHTDFIKSVTVIPCSPPLLLSTSSDRTCRLWDISEVLKGDGNARSSQILKEHTRPVECATWKADVDEDGKPMGTFTVWTGDSLGVIKKWKVEQGKLVYQEDVPGHETSVAKLIVAEEGLWSVSMDKMAILHPFTNASALTIPHNSYVKSILPLTSFALPNAHSLVLTGSEDEDIRVWDIESQPPRLKGTIQGHCAEVSDIKPYLRERDGKPELVVVSAGLDATLRTWTVQHILNPPELMYDETEEKDAVGLTEEEERELAELMSDEED | |||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| WDP7 | B11220 CNAG_07548 5’ flanking region primer L1 | ACCCTTTAGATAACGCACG |
| B11221 CNAG_07548 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACGGTTGTTTGAAAGAGGTGG | |
| B11222 CNAG_07548 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGGACATTGAATCACAACCCC | |
| B11223 CNAG_07548 3’ flanking region primer R2 | GACAGCGAGAACAAACAATC | |
| B11224 CNAG_07548 diagnostic screening primer SO, pairing with B79 | CAACAACGAACTCAAGACG | |
| B11225 CNAG_07548 Southern blot probe primer PO | GAGATAAAACGCACCTTGAG | |
| B12017 CNAG_07548 diagnostic screening primer SO2, paring with JOHE12579 | AAGGAAGGGATGGACGACTT | |
| B12018 CNAG_07548 Internal_LP | CAATGCGTAGACAGGCAAGA | |
| B12019 CNAG_07548 Internal_RP | GGGGAATGGTAAGTGCTGAA | |
| B5510 NAT#201 STM primer | CACCCTCTATCTCGAGAAAGCTCC | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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