Detailed Locus Information for CNAG_07951
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | WD-repeat protein JIP5 | |||||||||
| Gene Symbol | JIP5 | |||||||||
| NCBI Protein ID | XP_012047082.1 | |||||||||
| UniProt ID | T2BP49 | |||||||||
| KEGG Linkout | N/A | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MPDIKLKNQPFDVAFHPKESVVFSSLLTGQVCAWSYDDATGETSSSWSVRPSKRTARALSIEEDGDGIWMGGKSGSLFQLSTRDGSMTRERDNAHECPINRVYCVNRNIVASGDDDGVIKLWDPRQADSIRTYSQHFDYISDFTYFDDKRQLVATSGDGHLSVIDIRSNKSTPLTVSEDQEDELLSIVPIKGGQKAIVGSGLGILSVWNRQLGWADSVDRIPGHPASIDAIVALTPDVIATGSEDGMIRVIQVLPHKFLGVVATHEEFPVERIRLDRNNKWLGSVSHDECLKLTDVEDLFEDSDEDDEMEEDEPDSDEEKSKKKKKDNGMKDMSRGQAENDGSFFADL | |||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| JIP5 | B13665 CNAG_07951 5’ flanking region primer L1 | ACTCGGTTCGATGGTTGAAG |
| B13666 CNAG_07951 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACGGGCTTGGATGCTGGTATAA | |
| B13667 CNAG_07951 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGCAGATTCGGACGAGGAGAAG | |
| B13668 CNAG_07951 3’ flanking region primer R2 | GGGAGAGAAGCGTACGTTGA | |
| B13669 CNAG_07951 diagnostic screening primer SO, pairing with B79 | GCCCAGTGGAGGTAAGTCAA | |
| B13670 CNAG_07951 Southern blot probe primer PO | AGACACACGACCCTTTGGAC | |
| B22982 CNAG_07951 Internal_LP | GCTTTCAACTCGCGATGGAT | |
| B22983 CNAG_07951 Internal_RP | CAAAATGGAGTGTGGACGCA | |
| B22984 CNAG_07951 diagnostic screening primer SO2, paring with JOHE12579 | TAGCACACCTCTCCCACAAG | |
| B5520 NAT#220 STM primer | CAGATCTCGAACGATACCCA | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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