Detailed Locus Information for CNAG_01063
| Species | Cryptococcus neoformans var. grubii H99 | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | WD-repeat protein mip1 | ||||||||||||||||||
| Gene Symbol | Unknown | ||||||||||||||||||
| NCBI Protein ID | XP_012049073.1 | ||||||||||||||||||
| UniProt ID | J9VLF3 | ||||||||||||||||||
| KEGG Linkout | N/A | ||||||||||||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | ||||||||||||||||||
| Functional Annotation |
|
||||||||||||||||||
| Amino Acid Sequence | MTQYGETPSLPYARDESSTSQVQSAYPAYPAEAYEHQEAFVDEEDDFSADVDVGAALQYWTALRHMGGKGKQKEESEEDRTSKSPWRLRSKLKTVTAGLFICLNLGVDPPDIVKTNPCAKTECWIDPTQLPAPKAIDAIGRNLHQQFETLNPKVKYKAFLDPSIEDTKKNCISMRKTTKDERVVFYYNGHGVPRPTPSGEIWVFNKNYTQYIPVSLYDLQEWLGSPCIYIWECSAAGNILNNFMKSAERRDAEARHAVTQAGHEDLPRTSYTEALHLAACKANQILPMSPDLPADLFTCCLTSPIETALRHFVLQDPLRRNTGLSPDDPRSRITVDLAMRIPGDLKDRRTPLGELSWIFTAVTDTIAWLSFPRDMFNKLFRQDLLVAALFRNFLLAQRIMSAYHCTPTSIPEIPDTHNHPLWESWDLAVDACLAQLPGIFDQLGEPEPGKPLTIPPGMYKPSTFFAQHLQAFEVWLQHGATVSNKRTGRNRNDVVPRSPPEQLPIVLQVLLSQSHRLRALILISRFVDLGPWAVHLSLSIGIFPYVQKLLASPAIELKPVLIYIWARILAIDQSCQIDLLRDSGFTYFTQILAAYPQQGSLVIPNANEHRAMSSFILSILCRDFRQGQTACLNTHVFDACVARLHEDDWLLKTWCLLCIGQLWADYDEAKALFWQSQRQGELLSALRSTSVEVRAAALYAFGTLLGASAISIETPAKGGGGTGAQIGLSDVQQLELEAGVAFACMMSVKEDASPLVRKELVVVISCVVREWRGWLVCAAWVYYEQEAALVAAEDDKPMPKDVVSDTLEQWTMNEDKHPEEHQHNLTLLSSFKVLFETLLDLSVDPHTEVALTASTVVDYIIALLMDSPFVRVEGSAIRSIIRQHSRPQNAPRQATTAQLERSKLSHLNGVPPNLTRTSTDLDKSAVDNTSSLKRNSSVANALRSLASMTGFSAPETQSEEPLSTEPRQEIPTAPDLSTSAYISPYPEAGHERVQPDSHSASTSSTGRNPSSLKYLNTHMNTRSSLGILPSRLDQTPVEAYQVLEALTEEDMERLRMRRMRGREAGGDMDGRYGNNGLARSTDLGLGMVAKEVKDDVLPLRSGYFDWAMDYFRTPQMKAPDADEPGSQTYNEQAWKHQRNEQMVERSRASEEFAATHSWNMEAGTLHNDAWPLQLAFHSYDPVLAVTDDTDKICVWDWQNKTKLNKFSNQNIGGSSISSIHFVNEMASSLMLTASTEGSIRIFRDYDTPGETALASTFRAVSETYPVAHSSGLLTAWEQQKGHLLVGGDMKVVRLWDATVERHLRDIATQAGSNLTAISSDEPDGNVFVAGFGDGVVRLFDKRAEDAGEVVLRTWRQHKIWIQSVHLQKGSMRELVTGSMDGEVRVWDVRKPDEPLYTLPRRNEGLMALAVHTGAPVLARTTAITPYSTKQDLEITGFKNPLQPQRLAKISIPVPPAYNTPRHRAVDFLPSSASLVFHPVEMVVAAGGFDTSGTVKLYRCMAPDLPLG | ||||||||||||||||||
| AlphaFold Structure with WD40 domains |
|||||||||||||||||||
CTR4 Promoter Replacement Construction Design
| Gene Name | Primer Name | Sequence |
|---|---|---|
| KOG1 | B21036 CNAG_01063 5’ flanking region primer L1 | CCTCATCATCGTCGGCTAAT |
| B21037 CNAG_01063 5’ flanking region primer L2 | CACTCGAATCCTGCATGCGGCGAGTGCTACGTCGGC | |
| B21038 CNAG_01063 3’ flanking region primer R1 | CGACAACGACTTCACCAATCATGACCCAGTACGGAGAG | |
| B21039 CNAG_01063 3’ flanking region primer R2 | AGGCTGACGGGAATGTATTG | |
| B21040 CNAG_01063 diagnostic screening primer SO, pairing with B79 | TGAGGTATCCCAGGAAGTGC | |
| B21041 CNAG_01063 Southern blot probe primer PO | CCTCCTCATCCACAAATGCT |