Detailed Locus Information for CNAG_00693
| Species | Cryptococcus neoformans var. grubii H99 | ||||
|---|---|---|---|---|---|
| Gene Annotation | F-box and WD-40 domain-containing protein CDC4, variant | ||||
| Gene Symbol | CDC4 | ||||
| NCBI Protein ID | XP_012046895.1 | ||||
| UniProt ID | J9VEP9 | ||||
| KEGG Linkout | N/A | ||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | ||||
| Functional Annotation | |||||
| Amino Acid Sequence | MQGTPNTMDYNDLDSANGRALPIGGLTWSSEGPVLQMEDAVVETVVTHTTRTTTSFQPITLPRVPSPEHLKLPYHLSGEKYPLATQPAPSDMRLFTMTLGGRRVIVQDENAPGESGIEMSGPGWTRTLLNDSKTEVENGAEVVDESLTFLQALNRSKGKEKKREYEERSDAPHSPVANDISVRRSPPRKKIKGLDEINIPTGVNRALLSPLPSPEHEPIPLSSTAPTSQAPNMGSGVELSALFSLPSLASHFDALPDRLQQHFLMHLLRRARMPTLQRISNFVSTALRRDFITQLPREVAIQILKSVDGKSLASASRVCKKWKRIIDMERTVWKARLIDDKLWSGFGTEEQEESLVVERYEALDLHDQLEGRRTSYQAVSGEVEQMFSPTLGNDLPERPTPLKHVYRRRYQDQKNWIHTRPEHSSFTGHGTSVLTCLQFDEDKIVSASDDHSINIYNTSDGQLRKRLDGHEGGVWTLQYKGHTLVSGSTDRTVRIWDLEDLRMTYVFAGHTSTVRCLQIVEPVWEEETQSYQPPVPMIVTGSRDATLRVWKLPQKDDPLYDGIVEEEQTELIGPDINPFHMHLLEGHSLAVRAIATHGRICVSGSYDMSVRVWDIVKGTSLHVLTGHEAKVYSIVYDPYRKRCASGSMDSTVKVWDIVSGQCLHTLQGHTSLVGLLGLSPNYLVSAAADSSLRIWDPNTCQLKNVLASHSGAITCFQHDETKVVSGSDGSLKLWDVKTGTFVRDLVVGISSVWQVSFNKNLLVAASNRNGATVFDVFRFGQPSTQDVDDPSLDNLQPPRWERWAAELRKKLEMEEKKKRKSRSRKARTESAWDGRLEKARDPRLSSWKTALPYAHGYSVEGEESHEGAGLQPPPAFDFRAGSHTGSAKGNAQYQLSSAQLGSNHVVQRNLHDLSGSPTPTGVARRRTGPMISKVPRVEGSSGADAAMLPGSVVDPMYIGESSASTVTVEEDYQGDEGEEDEEMEDADEHSY | ||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| CDC4 (vari | B20252 CNAG_00693 5’ flanking region primer L1 | GCGGACAACGTGAAATGTG |
| B20253 CNAG_00693 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACGGCCATTTGCAGAATCTAGG | |
| B20254 CNAG_00693 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGGATGAGGGTGAGGAGGATGA | |
| B20255 CNAG_00693 3’ flanking region primer R2 | CGTCATTTGCACTGTCGAATA | |
| B20256 CNAG_00693 diagnostic screening primer SO, pairing with B79 | TGCATTAGCACCGTTCAGTC | |
| B20257 CNAG_00693 Southern blot probe primer PO | ACTCTCTCCAGGGGCATTTT | |
| B20339 CNAG_00693 Internal_LP | TGGAAGATCTCCGAATGACC | |
| B20340 CNAG_00693 Internal_RP | CTGGCCAAACCTGAAAACAT | |
| B20341 CNAG_00693 diagnostic screening primer SO2, paring with JOHE12579 | TTTTTGCCCTTTCCACAAAC | |
| B5502 NAT#125 STM primer | CGCTACAGCCAGCGCGCGCAAGCG | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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