Detailed Locus Information for CNAG_01439
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | U4/U6 small nuclear ribonucleoprotein PRP4 | |||||||||
| Gene Symbol | Unknown | |||||||||
| NCBI Protein ID | XP_012049544.1 | |||||||||
| UniProt ID | J9VQ13 | |||||||||
| KEGG Linkout | N/A | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MDLDDLITDNTYQLGGIDDERERAQNAAIIEELERKKRLRKLAVPTDDKKVRERLRAYGEPITLFGEGPGDRRDRLKYVQEQIEQAKGEGGMVVDESDESSDEDEEGEFYTEGSDDLLEARRKIARYSLSKARARIARQRREYSVPLGKLVNARKELFKELKSFNNFGSQFGDDRPLSTIRFSPSSQYILTTSWTGDTKLWDLPNLNPISTKRGHTEKVGGAAWHPFATVGASEEAVNFATGGGEGDVKLWSLSGEKPLSTLSGHTSRVGRLAFHPSGAYLASAGFDGTWRLWDVESSKELMTQEGHSKEVYAVGCQDDGALIASGGFDAIGRVWDLRTGRTAMVLDGHVKEILAMDFAPNGYQIATGSGDDTVRIWDLRALKTQYIIPAHKSSVADVKFFRSSGELPPIGIQGLPLSSRAAEVDGMDVDKSNDTEDKEKEERLSRSGMFLVTAGFDCNVRIWSADEWNMVRNLSTDAGKVMSVDVSGDGKFVASASYSRSFHLFGGENSL | |||||||||
| AlphaFold Structure with WD40 domains |
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CTR4 Promoter Replacement Construction Design
| Gene Name | Primer Name | Sequence |
|---|---|---|
| PRP4 | B17237 CNAG_01439 5’ flanking region primer L1 | GCCAGAAGACAAGTCGGAAG |
| B17238 CNAG_01439 5’ flanking region primer L2 | CACTCGAATCCTGCATGCCGCGGTGTGCAGGAGGAG | |
| B17239 CNAG_01439 3’ flanking region primer R1 | CGACAACGACTTCACCAATCATGGACTTGGACGACCTC | |
| B17240 CNAG_01439 3’ flanking region primer R2 | CTCCAACCTTCTCCGTGTGT | |
| B17241 CNAG_01439 diagnostic screening primer SO, pairing with B79 | GGCTCAGGGAACCATGAATA | |
| B17242 CNAG_01439 Southern blot probe primer PO | CTTCTTGCGTTCGAGCTCTT |