Detailed Locus Information for CNAG_00752
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | transducin family protein | |||||||||
| Gene Symbol | WDP12 | |||||||||
| NCBI Protein ID | XP_012046417.1 | |||||||||
| UniProt ID | J9VEZ6 | |||||||||
| KEGG Linkout | N/A | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MSLYDRQGEEEDFPHLNEDDIEEVLEDDGQRPVGLDDDDLGYGHDEDMKRLGDGIEGIEVIEKEPKENNSWGATALHQDHKSIFALSLHPKFPNPPLAITGGQDDTGFIFCPIPSSSADGNTSTFNSETFLPIRLSGHTDSVVTAAWNFDGDMVATGGMDGRVRVWRRVRKRRGQPLSEEVLNSLEGWRAWEFLTNLETGSEITWLTWHPKGNVLTAGCEDATAWMWNLPSGNTLAVLSSHTFSCTAGLFPPTAKNLLTASLDSSLILWDPRSPTPVWKSNIFLPPNSPELDPSIHGITSLAVSPRGDVVAVGGASGSVKIVSLSKGDVLTTLQGHRFGDSVEALCFVDLANGTGDGGRGLVCVSGGTDGCGFVWDVATGRVRSEIQHDEVITSLAPHPAPNQHLLTTASLDSTLKTWDIRTGILVATHLGHTGLVGGVAVASLGDGTVGVVSAGDEGISMIWRI | |||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| WDP12 | B17641 CNAG_00752 5’ flanking region primer L1 | GCAACTGGCTACAATGCAAG |
| B17642 CNAG_00752 5’ flanking region primer L2 | TGGCCGTCGTTTTACTTCCCTCGATACCATCTCCAA | |
| B17643 CNAG_00752 3’ flanking region primer R1 | TCATAGCTGTTTCCTGCTTGGTCATACGGGATTGGT | |
| B17644 CNAG_00752 3’ flanking region primer R2 | AAAGGGTGATGGGGAAGAGT | |
| B17645 CNAG_00752 diagnostic screening primer SO, pairing with B79 | TCCCTTTAACGCCGACACTA | |
| B17646 CNAG_00752 Southern blot probe primer PO | ACCTCCTGTTATTGCCAACG | |
| B17722 CNAG_00752 diagnostic screening primer SO2, paring with JOHE12579 | TCCATGCCAACCTCTACTCC | |
| B17867 CNAG_00752 Internal_LP | CTTTTGTCCCATTCCTTCCA | |
| B17868 CNAG_00752 Internal_RP | TGTCGCTACATCCCAAACAA | |
| B5514 NAT#210 STM primer | CTAGAGCCCGCCACAACGCT | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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