Detailed Locus Information for CNAG_05773
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | F-box and WD-40 domain-containing protein MET30 | |||||||||
| Gene Symbol | Unknown | |||||||||
| NCBI Protein ID | XP_012050517.1 | |||||||||
| UniProt ID | J9VPB2 | |||||||||
| KEGG Linkout | N/A | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MSPSAPPNMTAFPRENHYELEDQALDVIPTRAGRKLCVRHKQMANQNINEKLQRSLDSLNPSERAAITQMWSTFSTAPHGKRKIILEGILTMCCFSQLSHLSDSLNQIIRIDPFSLLPRETSLRILGYLDAFSLGRAAQVSRSWKALADDDLLWRRMCGQHIDRKCDKCGWGLPLLERKRLRVELKDRSPAGLVEHDHKHENENGESRLVTRDQVLSGNANTVSSIGGLKSCDTPAMYLFPPNVNAGTPKGIKRAAPESSIGAAKKVKVNGSDSDVVVVKPSSAGLTREVRLTRPWKTVYCERLMVERNWRKGRCSTKVLKGHTDGVMCLQYHTALTNPSYPVLITGSYDRTVRVWNLDTGEEVRVLRGHTRAVRALQFDQMLLFTGAMDGTVRMWNWRAGECLRVMDGHTDGVISLNYNGYLLATGSADSTINVWNFRTGNRFTLRGHEEWVNNVVLWDGKTSPSDTDPAAIPSFTQAVSNRCQKSKSPAAVSNEPTLPNIDPGAMLFSSSDDMTIKLWDLETATCIRTFEGHKAQVQSLRVLMVDMTEEEVAARDRRQRRQVTPSGTGFTATSLVSPPGSQAAIGPGSAAALDDAPAGTDPLEHHRGRSRSETVQPRVYVHSPDGTHSKSEREQSRGHEKKAIVASGSLDGTVKIWDVETGREQSTLFGHIEGVWAVDIDALRLVSASHDRTIKVWEKESAQCVQTLVGHRGAVTSLQLSDDMIVSGSDDGDVMIWNFASSSANNISNSANVGEPLVDITPSTTPAIV | |||||||||
| AlphaFold Structure with WD40 domains |
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CTR4 Promoter Replacement Construction Design
| Gene Name | Primer Name | Sequence |
|---|---|---|
| MET30 | B14454 CNAG_05773 5’ flanking region primer L1 | CAAACGCAAAATCCCAGACT |
| B14455 CNAG_05773 5’ flanking region primer L2 | CACTCGAATCCTGCATGCGGTGGGAGAGGTAAGGCC | |
| B14456 CNAG_05773 3’ flanking region primer R1 | ACAACGACTTCACCAATCATGTCTCCTTCTGCGCCA | |
| B14457 CNAG_05773 3’ flanking region primer R2 | CCCATTTTCATTTTCGTGCT | |
| B14458 CNAG_05773 diagnostic screening primer SO, pairing with B79 | GGAGAAGCGAATACGAGCAG | |
| B14459 CNAG_05773 Southern blot probe primer PO | GGTCAAAATGCCTTCCAAAA |