Detailed Locus Information for CNAG_00927
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | guanyl nucleotide binding protein | |||||||||
| Gene Symbol | SWT21 | |||||||||
| NCBI Protein ID | XP_012049200.1 | |||||||||
| UniProt ID | J9VL42 | |||||||||
| KEGG Linkout | N/A | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MEEQHHEQEQGQDEQHQAYAPPEYSFDNASLYASIDAASLSSPLENNFWRSARWSPDGSSVLTTTEDRSFRIHTLSESSLGTFETRQFKQPDSITSSNWFPTASSLIPESFCFVAGIRDNPVKLVDAKTGNVRASYPIIDHRERFISPHSLAFHPSLSKLYCGCSSFIEIIDLTSSSSTRLKTTFTRSSKDGQKGIISALAFAPDTTGSFVAGGYDGSVGMYMEDGDLEGWLGGVEGGGVTQLSYHPLNPTLLFVASRRSDIIQVYDLRNSSQPLHSLPRKGRTNQRLRFDIDVWGRWLVSGDEEGLVRIWDIISPEVPVVFEKKLHNDAVSSVQLHPYYPLLLTSSGSRNMTVSGSSLNNESSEGSQSDSDSDDSDSGLENENSKSMTTPRSKIKDSTMKVWSFLPSTTSI | |||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| SWT21 | B14281 CNAG_00927 5’ flanking region primer L1 | ACTGGCACCGTATGGAGAGT |
| B14282 CNAG_00927 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACTTTCGAGTGGTGAGGAAAGG | |
| B14283 CNAG_00927 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGTTTCGGGCTCATCTCTGAAT | |
| B14284 CNAG_00927 3’ flanking region primer R2 | ACGGTACGGGTCTTCTACGA | |
| B14285 CNAG_00927 diagnostic screening primer SO, pairing with B79 | TCCACCTGTGCGTCTTGTAG | |
| B14286 CNAG_00927 Southern blot probe primer PO | CATCAACGAGCTTGACAGGA | |
| B14511 CNAG_00927 diagnostic screening primer SO2, paring with JOHE12579 | GATGATCTCATCCCGAAGGA | |
| B14512 CNAG_00927 Internal_LP | ACTGGAAAGCGTAAGGCTGA | |
| B14513 CNAG_00927 Internal_RP | CGGCAGTATGGCCACTAAAT | |
| B5498 NAT#116 STM primer | GCACCCAAGAGCTCCATCTC | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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