Detailed Locus Information for CNAG_01013
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | chromatin binding protein | |||||||||
| Gene Symbol | SWD3 | |||||||||
| NCBI Protein ID | XP_012049255.1 | |||||||||
| UniProt ID | J9VR86 | |||||||||
| KEGG Linkout | N/A | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MAISRTDHPPTATRTPNYVPSSTLSAHSRAVTALRFSPDGTLLASAGADGWLHFWHPTSGQHIRGFRAHKAGINDITISPDSLYIATASDDHTSDIHLLHPTPGVTFYPPPLEPSDTSDTEDRPIVPVPPQASVSSSSTVPAIRHLVSHTAPVLSVAFSPKSNLLATGSFDESTIIWDVRRGKALRQLPAHADAVWCVAWDAEGEMVLTAGADGLIRLWDASTGQCLKTLDNDTNSPISHAAFTPSSVFLQASTLSSTLRIYNIHTGKVIKTIRAPGTFISERWPCPAVIYEGLPPLSQGSESNCHLDPPKEERMDVDSAERDPEPAKPPVAMPNVKTKMRDAWIISGSENGKLIIWDIQSKRVLQVLEGDLSHHCSVVALAVSPDGRTIASGSLEPEKVIKLWRDVE | |||||||||
| AlphaFold Structure with WD40 domains |
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Mutant Construction Design (Knockout)
| Gene Name | Primer Name | Sequence |
|---|---|---|
| SWD3 | B14675 CNAG_01013 5’ flanking region primer L1 | CTAAGAACCAGCAGCCCAAG |
| B14676 CNAG_01013 5’ flanking region primer L2 | TCACTGGCCGTCGTTTTACAGAAAATCGCAGAGCCGTAA | |
| B14677 CNAG_01013 3’ flanking region primer R1 | CATGGTCATAGCTGTTTCCTGGTCATTTAGATCCGCCGAAA | |
| B14678 CNAG_01013 3’ flanking region primer R2 | AATGTGTGGAAGGGCGTTAG | |
| B14679 CNAG_01013 diagnostic screening primer SO, pairing with B79 | TTGTGCCCTCGGATAAATTC | |
| B14680 CNAG_01013 Southern blot probe primer PO | AACTACCAGTCGCGAGGAGA | |
| B15832 CNAG_01013 Internal_LP | AATGCAGCGTGTGAGCTATG | |
| B15833 CNAG_01013 Internal_RP | ACATACGTGCCTCCTCAACC | |
| B15874 CNAG_01013 diagnostic screening primer SO2, paring with JOHE12579 | TCCATCACCATCATCCTTGA | |
| B5499 NAT#119 STM primer | CTCCCCACATAAAGAGAGCTAAAC | |
| B5532 STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Endoplasmic Reticulum Stress
Cell Wall Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Organ Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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